Identification of Proteins that Regulate Radiation-induced Apoptosis in Murine Tumors with Wild Type p53
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- SEONG Jinsil
- Department of Radiation Oncology, Yonsei University Medical College Brain Korea 21 Project for Medical Science, Yonsei University Medical College Institute for Gastroenterology, Yonsei University Medical College
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- OH Hae Jin
- Department of Radiation Oncology, Yonsei University Medical College Brain Korea 21 Project for Medical Science, Yonsei University Medical College
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- KIM Jiyoung
- Department of Radiation Oncology, Yonsei University Medical College Brain Korea 21 Project for Medical Science, Yonsei University Medical College
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- AN Jeung Hee
- Department of Radiation Oncology, Yonsei University Medical College Brain Korea 21 Project for Medical Science, Yonsei University Medical College
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- KIM Wonwoo
- Department of Radiation Oncology, Yonsei University Medical College Brain Korea 21 Project for Medical Science, Yonsei University Medical College
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In this study, we investigated the molecular factors determining the induction of apoptosis by radiation. Two murine tumors syngeneic to C3H/HeJ mice were used: an ovarian carcinoma OCa-I, and a hepatocarcinoma HCa-I. Both have wild type p53, but display distinctly different radiosensitivity in terms of specific growth delay (12.7 d in OCa-I and 0.3 d in HCa-I) and tumor cure dose 50% (52.6 Gy in OCa-I and > 80 Gy in HCa-I). Eight-mm tumors on the thighs of mice were irradiated with 25 Gy and tumor samples were collected at regular time intervals after irradiation. The peak levels of apoptosis were 16.1 ± 0.6% in OCa-I and 0.2 ± 0.0% in HCa-I at 4 h after radiation, and this time point was used for subsequent proteomics analysis. Protein spots were identified by peptide mass fingerprinting with a focus on those related to apoptosis. In OCa-I tumors, radiation increased the expression of cytochrome c oxidase and Bcl2/adenovirus E1B-interacting 2 (Nip 2) protein higher than 3-fold. However in HCa-I, these two proteins showed no significant change.<br> The results suggest that radiosensitivity in tumors with wild type p53 is regulated by a complex mechanism. Furthermore, these proteins could be molecular targets for a novel therapeutic strategy involving the regulation of radiosensitivity.<br>
収録刊行物
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- Journal of Radiation Research
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Journal of Radiation Research 48 (5), 435-441, 2007
Journal of Radiation Research 編集委員会
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詳細情報 詳細情報について
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- CRID
- 1390001205215216000
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- NII論文ID
- 110006388626
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- NII書誌ID
- AA00705792
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- ISSN
- 13499157
- 04493060
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- NDL書誌ID
- 8911905
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- NDL-Digital
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可