Nuclear accumulation and activation of nuclear factor κB after split-dose irradiation in LS174T cells
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- LIU Yong
- Department of Radiology, School of Medicine, Hirosaki University
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- NAKAHARA Takehisa
- Department of Radiological Technology, School of Health Science, Faculty of Medicine, Hirosaki University
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- MIYAKOSHI Junji
- Department of Radiological Technology, School of Health Science, Faculty of Medicine, Hirosaki University
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- HU Dong-Liang
- Department of Microbiology and Immunology, School of Medicine, Hirosaki University
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- NAKANE Akio
- Department of Microbiology and Immunology, School of Medicine, Hirosaki University
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- ABE Yoshinao
- Department of Radiology, School of Medicine, Hirosaki University
書誌事項
- タイトル別名
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- Nuclear Accumulation and Activation of Nuclear Factor .KAPPA.B after Split-dose Irradiation in LS174T Cells
- Nuclear accumulation and activation of nuclear factor kB after split dose irradiation in LS174T cells
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説明
Although radiation-induced gene expression has been extensively studied, most of the studies to date have focused on that after single-dose irradiation. As split-dose irradiation, rather than single-dose irradiation, is usual in clinical situations, we investigated the effects of split-dose irradiation on nuclear factor κB (NF-κB) in the human rectum carcinoma cell line, LS174T. After either single- or split-dose irradiation with a different interval, nuclear localization of NF-κB was examined by Western blot and immunofluorescence and its DNA-binding activity was measured by ELISA-based assay. Irradiation-induced NF-κB nuclear accumulation and DNA binding activity increased in a dose-dependent manner. The peak of NF-κB nuclear accumulation and DNA binding activity was seen 2 to 6 hours after a single dose of 4 Gy irradiation and returned to control levels after 12 hours. In split-dose irradiation, NF-κB activity was similar after the first and second doses of 4 Gy irradiation separated by 12 hours. In addition, NF-κB activity was decreased by lengthening the interval between irradiation. The cell survival, which was assessed by colony formation assay, showed inverse correlation to this: the surviving fraction was higher after split-dose irradiation than after single-dose irradiation of the same total dose and it increased as the interval between irradiation was lengthened. Thus the present results showed a correlation between NF-κB activation and the repair of sublethal damage in split-dose irradiation.<br>
収録刊行物
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- Journal of Radiation Research
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Journal of Radiation Research 48 (1), 13-20, 2007
Journal of Radiation Research 編集委員会
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詳細情報 詳細情報について
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- CRID
- 1390001205216214784
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- NII論文ID
- 110006570512
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- NII書誌ID
- AA00705792
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- DOI
- 10.1269/jrr.0615
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- ISSN
- 13499157
- 04493060
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- NDL書誌ID
- 8748493
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- NDL-Digital
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可