Nuclear accumulation and activation of nuclear factor κB after split-dose irradiation in LS174T cells

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  • LIU Yong
    Department of Radiology, School of Medicine, Hirosaki University
  • NAKAHARA Takehisa
    Department of Radiological Technology, School of Health Science, Faculty of Medicine, Hirosaki University
  • MIYAKOSHI Junji
    Department of Radiological Technology, School of Health Science, Faculty of Medicine, Hirosaki University
  • HU Dong-Liang
    Department of Microbiology and Immunology, School of Medicine, Hirosaki University
  • NAKANE Akio
    Department of Microbiology and Immunology, School of Medicine, Hirosaki University
  • ABE Yoshinao
    Department of Radiology, School of Medicine, Hirosaki University

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タイトル別名
  • Nuclear Accumulation and Activation of Nuclear Factor .KAPPA.B after Split-dose Irradiation in LS174T Cells
  • Nuclear accumulation and activation of nuclear factor kB after split dose irradiation in LS174T cells

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Although radiation-induced gene expression has been extensively studied, most of the studies to date have focused on that after single-dose irradiation. As split-dose irradiation, rather than single-dose irradiation, is usual in clinical situations, we investigated the effects of split-dose irradiation on nuclear factor κB (NF-κB) in the human rectum carcinoma cell line, LS174T. After either single- or split-dose irradiation with a different interval, nuclear localization of NF-κB was examined by Western blot and immunofluorescence and its DNA-binding activity was measured by ELISA-based assay. Irradiation-induced NF-κB nuclear accumulation and DNA binding activity increased in a dose-dependent manner. The peak of NF-κB nuclear accumulation and DNA binding activity was seen 2 to 6 hours after a single dose of 4 Gy irradiation and returned to control levels after 12 hours. In split-dose irradiation, NF-κB activity was similar after the first and second doses of 4 Gy irradiation separated by 12 hours. In addition, NF-κB activity was decreased by lengthening the interval between irradiation. The cell survival, which was assessed by colony formation assay, showed inverse correlation to this: the surviving fraction was higher after split-dose irradiation than after single-dose irradiation of the same total dose and it increased as the interval between irradiation was lengthened. Thus the present results showed a correlation between NF-κB activation and the repair of sublethal damage in split-dose irradiation.<br>

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