An alkaline separation method for detection of small amount of DNA damage.

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  • SAKAI KAZUO
    Department of Radiation Biophysics, Faculty of Medicine University of Tokyo
  • OKADA SHIGEFUMI
    Department of Radiation Biophysics, Faculty of Medicine University of Tokyo

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An alkaline separation technique originally established by Ahnstrom is modified to detect small amount of DNA damage in X-irradiated mouse leukemic L5178Y cells. It is made quantitative by calibration with an alkaline sucrose gradient centrifugation. The present method would make it possible to study DNA damage and its repair within a dose range of X-rays where cell survival and mutation are usually investigated. It is also useful for detecting DNA damage caused by chemicals.

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