Triphasic Low-dose Response in Zebrafish Embryos Irradiated by Microbeam Protons
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- CHOI Viann Wing Yan
- Department of Physics and Materials Science, City University of Hong Kong
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- YUM Emily Hoi Wa
- Department of Physics and Materials Science, City University of Hong Kong
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- KONISHI Teruaki
- Department of Technical Support and Development, National Institute of Radiological Sciences
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- OIKAWA Masakazu
- Department of Technical Support and Development, National Institute of Radiological Sciences
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- CHENG Shuk Han
- Department of Biology and Chemistry, City University of Hong Kong State Key Laboratory in Marine Pollution, City University of Hong Kong
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- YU Kwan Ngok
- Department of Physics and Materials Science, City University of Hong Kong State Key Laboratory in Marine Pollution, City University of Hong Kong
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The microbeam irradiation system (Single-Particle Irradiation System to Cell, acronym as SPICE) at the National Institute of Radiological Sciences (NIRS), Japan, was employed to irradiate dechorionated zebrafish embryos at the 2-cell stage at 0.75 h post fertilization (hpf) by microbeam protons. Either one or both of the cells of the embryos were irradiated with 10, 20, 40, 50, 80, 100, 160, 200, 300 and 2000 protons each with an energy of 3.37 MeV. The embryos were then returned back to the incubator until 24 hpf for analyses. The levels of apoptosis in zebrafish embryos at 25 hpf were quantified through terminal dUTP transferase-mediated nick end-labeling (TUNEL) assay, with the apoptotic signals captured by a confocal microscope. The results revealed a triphasic dose-response for zebrafish embryos with both cells irradiated at the 2-cell stage, namely, (1) increase in apoptotic signals for < 200 protons (< 30 mGy), (2) hormesis to reduce the apoptotic signals below the spontaneous number for 200–400 protons (at doses of 30–60 mGy), and (3) increase in apoptotic signals again for > 600 protons (at doses > 90 mGy). The dose response for zebrafish embryos with only one cell irradiated at the 2-cell stage was also likely a triphasic one, but the apoptotic signals in the first zone (< 200 protons or < 30 mGy) did not have significant differences from those of the background. At the same time, the experimental data were in line with induction of radiation-induced bystander effect as well as rescue effect in the zebrafish embryos, particular in those embryos with unirradiated cells.
収録刊行物
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- Journal of Radiation Research
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Journal of Radiation Research 53 (3), 475-481, 2012
Journal of Radiation Research 編集委員会
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詳細情報 詳細情報について
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- CRID
- 1390001205217192320
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- NII論文ID
- 130001876863
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- NII書誌ID
- AA00705792
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- COI
- 1:STN:280:DC%2BC38rks1aiuw%3D%3D
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- ISSN
- 13499157
- 04493060
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- NDL書誌ID
- 023682902
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- PubMed
- 22498889
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可