Changes in the Viability and Optimal Moisture Content of Beech (Fagus crenata BL.) Seeds during 10 Years of Cold Storage.

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  • 冷凍貯蔵したブナ種子の発芽率と含水率の１０年間の変化
  • レイトウ チョゾウ シタ ブナ シュシ ノ ハツガリツ ト ガンスイリツ ノ 10ネンカン ノ ヘンカ

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The germination capacity of stored seeds of Fagus crenata was tested to identify long-term (5+ years) storage conditions. Seeds with three different moisture levels (moisture content [MC] of 11.3, 6.1, and 4.0%) were stored at −20ºC. The percentage germination of seeds with 11.3% MC decreased after 4 years and reached zero after 6 years. Seeds with 4.0% MC maintained their germination capacity for at least 4 years, and seeds with 6.1% MC exhibited high germination percentages (60%) after 5 years of storage. Although the germination capacity began to decrease after 6 years, 25% of beech seeds showed some germination capabilities even after 10 years of storage. These results were supported by a follow-up experiment using seeds with 6.6% MC at −20ºC. In the follow-up experiment, seeds maintained high germination percentages (60%) after 7 years of storage. These findings indicated that the optimal MC of F. crenata seeds falls between 6% and 9%. The MC of seeds in the first experiment slightly increased (from 6.1% to 7.1%) at the end of 8 years of storage. Seedling malformation was observed in 8% to 25% of germinated seedlings after 4 years of storage; however, malformation did not affect seedling development. Further tests are required to confirm whether this seed storage method is widely applicable for seeds produced in the other distribution range of F. crenata.

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