Use of Self-incompatibility Gene and Nuclear SSRs as a Molecular Tool to Detect the Genome Derived from Prunus yedoensis "Someiyoshino".

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  • ソメイヨシノ由来のゲノムを検出するマーカー・ツールとしての自家不和合性遺伝子と核ＳＳＲの利用
  • ソメイヨシノ ユライ ノ ゲノム オ ケンシュツ スル マーカー ツール ト シテ ノ ジカ フワ アイショウ イデンシ ト カク SSR ノ リヨウ

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The large scale planting of cultivated species such as Prunus yedoensis “Someiyoshino” can disturb the genetic structure of wild Prunus species. We developed genetic markers to detect the gene flow from P. yedoensis to wild Prunus species. The self-incompatibility gene (S-gene) was used as the genetic markers because it is highly polymorphic. We designed the PCR primer pairs to amplify the two S-alleles of P. yedoensis specifically. Although the genetic markers developed in this study almost successfully detected the pollen-mediated gene flow from P. yedoensis, it seemed difficult to accurately discriminate the gene flow from the trees of wild Prunus species, which already had one or two copies of same S-alleles with P. yedoensis’s. If combined with the SSR markers, samples with genome surely derived from P. yedoensis could be determined. Thus, our approach based on the S-locus polymorphism has a practical use, and the developed genetic markers are powerfully available as a molecular tool to evaluate the dispersal pattern of the genome derived from P. yedoensis.

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