Application of a Modified MS Medium for Tissue Culture with Cutting in Phalaenopsis－Comparison with Other Conventional Media with Regard to the Survival Rate and Varietal Differences in Cultural Characteristics－

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  • 切断を伴うコチョウランの微細繁殖への修正ムラシゲ・スクーク培地の適用－他培地との生存率の比較と培養特性の品種間差－
  • セツダン オ トモナウ コチョウラン ノ ビサイ ハンショク エ ノ シュウセイ ムラシゲ ・ スクークバイチ ノ テキヨウ : タバイチ ト ノ セイゾンリツ ノ ヒカク ト バイヨウ トクセイ ノ ヒンシュ カンサ

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Abstract

Cut tissues of Phalaenopsis elute a lot of phenolic compounds in the process of wound response, and the toxicity of these phenolic compounds damage the tissues. A newly modified Murashige and Skoog (MS) medium, which we have named Phalaenopsis shoot reproduction (PSR) medium, was developed to avoid such damages, and it is suitable for micropropagation with tissue cutting. Shoot survival rates and eluted phenolic compounds were examined when shoots (length, 2 cm) that were cut off from PLBs propagated on PSR medium were grown on PSR medium, modified VW medium, and NDM. The survival rate of shoots was much lower on modified VW medium than on the other media. In addition, shoots on PSR medium formed vigorous leaves and roots; thus PSR medium is the best for shoot growth. Many ethanol-soluble phenolic compounds were eluted on PSR medium, and many water-soluble ones were eluted on NDM. We applied the adventitious bud proliferation method using PSR medium to major varieties and their wild ancestor species and studied the varietal differences in cultural characteristics. This method was easily applicable to almost all species, including all the examined hybrids and wild species of the subgenus Phalaenopsis that is an important group as hybrid parents. It was shown that PSR medium is suitable for the culture of Phalaenopsis with cutting and that this proliferation method can be used for a large majority of Phalaenopsis varieties.

Journal

  • Shokubutsu Kankyo Kogaku

    Shokubutsu Kankyo Kogaku 26 (2), 109-117, 2014

    Japanese Society of Agricultural, Biological and Environmental Engineers and Scientists

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