Inhibitory effect of constituents of Bu-Shen-Jian-Gu-Tang on osteoclast-like cell formation
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- SHI Liying
- Institute of Natural Medicine, University of Toyama
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- TEZUKA Yasuhiro
- Institute of Natural Medicine, University of Toyama
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- MIYAHARA Tatsuro
- Faculty of Medicine, University of Toyam
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- YIN Jun
- School of Traditional Chinese Medicines, Shenyang Pharmaceutical University
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- NOBUKAWA Takahiro
- Kanazawa Medical University
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- KADOTA Shigetoshi
- Institute of Natural Medicine, University of Toyama
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Abstract
The constituents of Bu-Shen-Jian-Gu-Tang and their inhibitory activities on osteoclast-like cell formation were investigated. Bu-Shen-Jian-Gu-Tang was fractionated by a Diaion HP-20 column with EtOH-H2O, and the 60% and 90% EtOH-H2O fractions showed more potent inhibitory activities on osteoclast-like cell formation and bone resorption. Chemical investigation of these two fractions resulted in the isolation of 11 compounds, for which the structures were elucidated based on spectroscopic analysis and chemical reactions. The inhibitory activities of the isolated compounds on osteoclast-like cell formation were evaluated. Among the eleven compounds, icariin (1), icarisid II (2), 2"-O-rhamnosylicariside II (3), kaempferol (8), chikusetsusaponin IVa (10) and chikusetsusaponin V (11) showed potent inhibitory activities at a concentration of 2 μM without toxic effects. The preventive effect of Bu-Shen-Jian-Gu-Tang on bone loss may be due to the inhibitory activities of these compounds.
Journal
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- Journal of Traditional Medicines
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Journal of Traditional Medicines 24 (1), 19-23, 2007
Medical and Pharmaceutical Society for WAKAN-YAKU
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Keywords
Details 詳細情報について
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- CRID
- 1390001205233153024
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- NII Article ID
- 110006224306
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- NII Book ID
- AA12035198
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- ISSN
- 18813747
- 18801447
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- HANDLE
- 10110/1992
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- NDL BIB ID
- 8743224
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- Text Lang
- en
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- Data Source
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- JaLC
- IRDB
- NDL
- CiNii Articles
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- Abstract License Flag
- Disallowed