γ-Cyclodextrin/polyurethane Copolymer Adsorbent for Selective Removal of Endotoxin from DNA Solution
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- Sakata Masayo
- Department of Applied Chemistry & Biochemistry, Graduate School of Science and Technology, Kumamoto University
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- Kimura Kasane
- Department of Applied Chemistry & Biochemistry, Graduate School of Science and Technology, Kumamoto University
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- Uezono Koji
- Department of Applied Chemistry & Biochemistry, Graduate School of Science and Technology, Kumamoto University
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- Todokoro Masami
- JNC Corporation, Yokohama Research Center
Bibliographic Information
- Other Title
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- 包接能を利用した内毒素吸着剤の開発
- 抄録 包接能を利用した内毒素吸着剤の開発
- ショウロク ホウセツノウ オ リヨウ シタ ナイドクソ キュウチャクザイ ノ カイハツ
- γ-Cyclodextrin/polyurethane Copolymer Adsorbent for Selective Removal of Endotoxin from DNA Solution
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Abstract
Copolymer particles for removal of endotoxins (lipopolysaccharides; LPS) were prepared by suspension copolymerization of γ-cyclodextrin (CyD) and 1, 6-hexamethylenediisocyanate. The LPS-removing activity of the copolymer particles was compared with that of poly (ε-lysine) -Cellufine (cationic adsorbent) by a batch method. LPS-removing activity of the cationic adsorbent was unsatisfactory because both the DNA and the LPS were adsorbed at pH 6.0, ionic strength of μ=0.05-0.8. In contrast, the copolymer particles with γ-CyD cavity (CyD content: 14–20 mol%) could selectively remove LPS from a DNA solution (50 μg/ml, pH 6.0 and μ=0.05–0.2) containing LPS (15 EU/ml). The residual concentration of LPS in the treated DNA solution was below 0.1 EU/ml, and the recovery of DNA was 99%.
Journal
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- Journal of The Society of Japanese Women Scientists
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Journal of The Society of Japanese Women Scientists 15 (1), 28-32, 2015
The Society of Japanese Women Scientists
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Details 詳細情報について
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- CRID
- 1390001205234045056
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- NII Article ID
- 130005063871
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- NII Book ID
- AA12034071
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- ISSN
- 21863776
- 13494449
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- NDL BIB ID
- 026859572
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- Text Lang
- ja
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed