Evaluation of immunoglobulin G antibody titer measurement in the simplified test for multiple bacterial lnfection in periodontal disease based on self-sampling of fingertip capillary blood: focusing on Porphyromonas gingivalis antigen

DOI Web Site 被引用文献1件
  • Maehata Eisuke
    Division of Endocrinology and Metabolism, Department of Internal Medicine, Showa University Fujiv_aoka Hospital
  • Maehata Yojiro
    Division of Pharmacology, Kanagawa Dental College
  • Lee Masaichi-Cong-il
    Division of Pharmacology, Kanagawa Dental College
  • Kudo Chieko
    Department of Pathophysiology-Periodontal Science, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
  • Takashiba Shogo
    Department of Pathophysiology-Periodontal Science, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
  • Shimomura Hiroji
    Faculty of Health Science Technology, Bunkyo Gakuin University
  • Yamakado Minoru
    Mitsui Memorial Hospital
  • Yano Masao
    Mitsui Memorial Hospital
  • Shiba Teruo
    Mitsui Memorial Hospital
  • Hatakeyama Ikuo
    Funabashi Municipal Medical Center
  • Inoue Minoru
    PL Health Care Center
  • Kouka Kunio
    Saiseikai Central Hospital
  • Adachi Tetsuo
    Laboratory of Clinical Pharmaceutics, Gifu Pharmaceutical University
  • Kishikawa Naoya
    Division of Analytical Chemistry, Course of Pharmaceutical Sciences Department of Environmental and Pharmaceutical Sciences Graduate School of Biomedical Sciences, Nagasaki University
  • Kuroda Naotaka
    Division of Analytical Chemistry, Course of Pharmaceutical Sciences Department of Environmental and Pharmaceutical Sciences Graduate School of Biomedical Sciences, Nagasaki University
  • Sugimoto Shinya
    Leisure, Inc., Nagasaki Laboratory
  • Watanabe Hiromi
    Leisure, Inc., Nagasaki Laboratory
  • Koga Kazumasa
    Leisure, Inc., Nagasaki Laboratory
  • Ikoshi Naoko
    The Tokyo College of Medical Technology
  • Shimizu Katsuhisa
    Wales, Natural Animal Centre.

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タイトル別名
  • Evaluation of Immunoglobulin G Antibody Titer Measurement in the Simplified Test for Multiple Bacterial Infection in Periodontal Disease Based on Self-Sampling of Fingertip Capillary Blood
  • -Focusing on Porphyromonas gingivalis Antigen-

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Background Periodontal disease is a multiple infection caused by bacteria represented by Porphyromonas gingivalis (Pg). The prevalence of periodontal disease is high, as it predominantly affects the people in the same age range as diabetes mellitus, but it is often overlooked because of the lack of subjective symptoms. There has been a need for the introduction of self-sampled device-treated plasma using fingertip capillary blood in routine tests.<br>Methods Based on the report by Kudo et al. (Beppu Conference 2006, p.46, 2006) on enzyme-linked immunosorbent assay (ELISA) as a blood test for periodontal disease bacteria, the precision and disease specificity were examined towards the establishment of a methodology for routine tests.<br>Results The intra-assay reproducibility of the measurements using Pg and 3 other types of antigens, Prevotella intermedia (Pi), Eikenella corroders (Ec), and Actinobacillus actinomycetemcomitans (Aa), was coefficient of variation (CV) 4-7%, and the results from capillary blood and those from venous blood were closely analogous to each other with a correlation (r) of 0.900 or more. The difference between the non-periodontal control subjects group and the periodontal disease group in the distribution of data on histograms using the Pg antigen was approximately 7-fold. In addition, the correlation with mean periodontal pocket depth was significant (r=0.597, p<0.001) in the group showing the test results (SD index; SDI) of 20 or more. These results substantiated the specificity of this method.<br>Conclusion The ability to detect periodontal disease in the setting of “Human Dry Dock” screening examinations using the sampling and test methods proposed by us would be an important means to improve services. This study established the practical feasibility of this approach.

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