Oxidative DNA Damage Induced by 364-nm UVA Laser in Yeast Cells
-
- Negishi Kazuo
- Okayama University Advanced Science Research Center, Dentistry and Pharmaceutical Sciences
-
- Higashi Shoichi
- Okazaki National Research Institutes, National Institute for Basic Biology
-
- Nakamura Takanori
- Okazaki National Research Institutes, National Institute for Basic Biology
-
- Otsuka Chie
- Research Institute for Biological Sciences
-
- Watanabe Masakatsu
- Okazaki National Research Institutes, National Institute for Basic Biology
-
- Negishi Tomoe
- Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Search this article
Description
The mechanisms of the toxic effects of UVA (320-400 nm) irradiation remain unclear. The actions of monochromatic longer wavelength UVA, in particular, have been difficult to analyze because of a lack of a powerful light source; however, a UVA laser that can be used for biological studies was recently developed. In the current studies, we examined the effects of 364-nm irradiation on yeast cells using a potent UVA laser. We found that, when irradiated under aerobic conditions, yeast cells lacking Ogg1 glycosylase were more sensitive than those with Ogg1. The ability of the 364-nm light to kill the yeast cells was almost eliminated by purging with argon gas. The mutagenic effects of the UVA irradiation did not appear to be enhanced by a lack of Ogg1. These results indicate the killing of yeast cells by 364-nm UVA may be dependent on oxidation and may involve DNA lesions that can be repaired by Ogg1.<br>
Journal
-
- Genes and Environment
-
Genes and Environment 28 (2), 74-76, 2006
The Japanese Environmental Mutagen Society
- Tweet
Details 詳細情報について
-
- CRID
- 1390001205257034496
-
- NII Article ID
- 110004749522
-
- NII Book ID
- AA1212552X
-
- ISSN
- 18807062
- 18807046
-
- NDL BIB ID
- 7945479
-
- Text Lang
- en
-
- Data Source
-
- JaLC
- NDL Search
- Crossref
- CiNii Articles
- OpenAIRE
-
- Abstract License Flag
- Disallowed