Oxidative DNA Damage Induced by 364-nm UVA Laser in Yeast Cells
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- Negishi Kazuo
- Okayama University Advanced Science Research Center, Dentistry and Pharmaceutical Sciences
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- Higashi Shoichi
- Okazaki National Research Institutes, National Institute for Basic Biology
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- Nakamura Takanori
- Okazaki National Research Institutes, National Institute for Basic Biology
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- Otsuka Chie
- Research Institute for Biological Sciences
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- Watanabe Masakatsu
- Okazaki National Research Institutes, National Institute for Basic Biology
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- Negishi Tomoe
- Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
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Abstract
The mechanisms of the toxic effects of UVA (320-400 nm) irradiation remain unclear. The actions of monochromatic longer wavelength UVA, in particular, have been difficult to analyze because of a lack of a powerful light source; however, a UVA laser that can be used for biological studies was recently developed. In the current studies, we examined the effects of 364-nm irradiation on yeast cells using a potent UVA laser. We found that, when irradiated under aerobic conditions, yeast cells lacking Ogg1 glycosylase were more sensitive than those with Ogg1. The ability of the 364-nm light to kill the yeast cells was almost eliminated by purging with argon gas. The mutagenic effects of the UVA irradiation did not appear to be enhanced by a lack of Ogg1. These results indicate the killing of yeast cells by 364-nm UVA may be dependent on oxidation and may involve DNA lesions that can be repaired by Ogg1.<br>
Journal
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- Genes and Environment
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Genes and Environment 28 (2), 74-76, 2006
The Japanese Environmental Mutagen Society
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Details
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- CRID
- 1390001205257034496
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- NII Article ID
- 110004749522
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- NII Book ID
- AA1212552X
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- ISSN
- 18807062
- 18807046
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- NDL BIB ID
- 7945479
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed