Oxidative DNA Damage Induced by 364-nm UVA Laser in Yeast Cells

  • Negishi Kazuo
    Okayama University Advanced Science Research Center, Dentistry and Pharmaceutical Sciences
  • Higashi Shoichi
    Okazaki National Research Institutes, National Institute for Basic Biology
  • Nakamura Takanori
    Okazaki National Research Institutes, National Institute for Basic Biology
  • Otsuka Chie
    Research Institute for Biological Sciences
  • Watanabe Masakatsu
    Okazaki National Research Institutes, National Institute for Basic Biology
  • Negishi Tomoe
    Graduate School of Medicine, Dentistry and Pharmaceutical Sciences

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Abstract

The mechanisms of the toxic effects of UVA (320-400 nm) irradiation remain unclear. The actions of monochromatic longer wavelength UVA, in particular, have been difficult to analyze because of a lack of a powerful light source; however, a UVA laser that can be used for biological studies was recently developed. In the current studies, we examined the effects of 364-nm irradiation on yeast cells using a potent UVA laser. We found that, when irradiated under aerobic conditions, yeast cells lacking Ogg1 glycosylase were more sensitive than those with Ogg1. The ability of the 364-nm light to kill the yeast cells was almost eliminated by purging with argon gas. The mutagenic effects of the UVA irradiation did not appear to be enhanced by a lack of Ogg1. These results indicate the killing of yeast cells by 364-nm UVA may be dependent on oxidation and may involve DNA lesions that can be repaired by Ogg1.<br>

Journal

  • Genes and Environment

    Genes and Environment 28 (2), 74-76, 2006

    The Japanese Environmental Mutagen Society

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Details

  • CRID
    1390001205257034496
  • NII Article ID
    110004749522
  • NII Book ID
    AA1212552X
  • DOI
    10.3123/jemsge.28.74
  • ISSN
    18807062
    18807046
  • NDL BIB ID
    7945479
  • Text Lang
    en
  • Data Source
    • JaLC
    • NDL
    • Crossref
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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