Accurate Measurement of 8-OH-dG and 8-OH-Gua in Mouse DNA, Urine and Serum: Effects of X-ray Irradiation
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- Kawai Kazuaki
- Department of Environmental Oncology, Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health
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- Li Yun-Shan
- Department of Environmental Oncology, Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health
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- Kasai Hiroshi
- Department of Environmental Oncology, Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health
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Oxidative stress is believed to increase the risk of lifestyle-related diseases, such as cancer and heart disease. For the measurement of oxidative stress in vivo, 8-hydroxydeoxyguanosine (8-OH-dG) in DNA or urine is the most popular biomarker. However, there are some difficulties in the reproducible and accurate analysis of 8-OH-dG in DNA. In this study, we found that artifactual 8-OH-dG was elevated significantly with time in an analytical sample kept at 10°C, but not for one kept at -80°C. Furthermore, we developed a method for urinary 8-OH-dG analysis with high accuracy by an HPLC-ECD system, using anion-exchange- and reverse-phase-columns. This method can also be used for urinary and serum 8-hydroxyguanine (8-OH-Gua, free base) analyses, with a slight modification. By applying these improved methods, we confirmed the induction of oxidative stress with low dose (2 Gy) whole body X-ray irradiation of mice. The 8-OH-dG levels in the mouse urine were increased about 4.2-fold by 2 Gy irradiation, in a dose-dependent manner. The 8-OH-Gua levels in the serum were also increased with 2 Gy of irradiation. These results suggest that our improved 8-OH-dG and 8-OH-Gua analysis methods are useful for measurements of oxidative stress in vivo.<br>
収録刊行物
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- Genes and Environment
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Genes and Environment 29 (3), 107-114, 2007
日本環境変異原学会
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詳細情報 詳細情報について
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- CRID
- 1390001205257146240
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- NII論文ID
- 130004481159
- 110006391305
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- NII書誌ID
- AA1212552X
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- ISSN
- 18807062
- 18807046
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- NDL書誌ID
- 8886751
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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- 使用不可