New Method of Specimen Preparation as Cell Suspension for a Micronucleus Test
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- Kawabata Masayoshi
- Kashima Laboratory, Mitsubishi Chemical Safety Institute Ltd.
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- Takasawa Hironao
- Kashima Laboratory, Mitsubishi Chemical Safety Institute Ltd.
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- Minowa Shigenori
- Kashima Laboratory, Mitsubishi Chemical Safety Institute Ltd.
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- Saitou Hitoshi
- Kashima Laboratory, Mitsubishi Chemical Safety Institute Ltd.
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- Nakagawa Munehiro
- Kashima Laboratory, Mitsubishi Chemical Safety Institute Ltd.
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- Nakajima Madoka
- Biosafety Research Center, Foods, Drugs and Pesticides
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- Hamada Shuichi
- Kashima Laboratory, Mitsubishi Chemical Safety Institute Ltd.
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抄録
A new specimen preparation method named “cell suspension method” for in vivo micronucleus test is proposed. Generally, a specimen of the micronucleus test is prepared by smearing the sample cells on a slide glass before staining with Giemsa or acridine orange (A.O.). In this new method, the sample cells are suspended with formalin, and stained with A.O., then put on a slide glass and examined under a microscope without smearing. This method is more convenient than the conventional method in consideration of preparation and observation of specimens. Furthermore, preparation is repeatable after the observation in this method, the specimen is suitable for a long storage, and this method is applicable to specimen preparation using peripheral blood.<br>
収録刊行物
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- Genes and Environment
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Genes and Environment 29 (3), 100-106, 2007
日本環境変異原学会
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詳細情報 詳細情報について
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- CRID
- 1390001205257149056
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- NII論文ID
- 110006391304
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- NII書誌ID
- AA1212552X
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- ISSN
- 18807062
- 18807046
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- NDL書誌ID
- 8886736
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可