DNA Polymerase λ Promotes Mutagenesis Induced by 8-Oxo-7,8-dihydroguanine (8-hydroxyguanine) Paired with Adenine
-
- Kamiya Hiroyuki
- Graduate School of Science and Engineering, Ehime University
-
- Kurokawa Masahiro
- Graduate School of Science and Engineering, Ehime University
Bibliographic Information
- Other Title
-
- DNA Polymerase ^|^lambda; Promotes Mutagenesis Induced by 8-Oxo-7,8-dihydroguanine (8-hydroxyguanine) Paired with Adenine
Search this article
Description
DNA polymerase λ preferentially inserts dCTP opposite 8-oxo-7,8-dihydroguanine (GO, 8-hydroxyguanine) in vitro, and this function is considered to be important after A base removal from GO:A pairs by the MUTYH DNA glycosylase. However, dCTP incorporation would promote A:T→C:G transversion mutations induced by 8-oxo-7,8-dihydro-dGTP (dGOTP), the mutation triggered by the incorporation of dGOTP opposite A in the template DNA. In this study, double-stranded plasmid DNA containing the GO:A pair, an intermediate in the dGOTP mutagenesis pathway, was transfected into human cells in which DNA polymerase λ was knocked down. The knockdown of DNA polymerase λ significantly reduced the frequency of A:T→C:G transversion mutations induced by the GO:A pair although the knockdown effect was small. These results suggested that DNA polymerase λ is involved in the mutagenesis processes of GO, generated by dGOTP, in the nucleotide pool.<br>
Journal
-
- Genes and Environment
-
Genes and Environment 35 (4), 105-109, 2013
The Japanese Environmental Mutagen Society
- Tweet
Details 詳細情報について
-
- CRID
- 1390001205257244032
-
- NII Article ID
- 10031196752
-
- NII Book ID
- AA1212552X
-
- COI
- 1:CAS:528:DC%2BC3sXhvVyhurvJ
-
- ISSN
- 18807062
- 18807046
-
- NDL BIB ID
- 025004111
-
- Text Lang
- en
-
- Data Source
-
- JaLC
- NDL
- Crossref
- CiNii Articles
- KAKEN
-
- Abstract License Flag
- Disallowed