Studies on the assimilation of nitrites by fungi Part II.

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(1) A method for the estimation of nitrite in presence of nitrates is devised.<BR>(2) In the medium, which contains natrium nitrate as its sole nitrogen source, the nitrite is formed by Asp. Oryzae A, Takahashi.<BR>(3) The Oxidation of the Nanitrite to the nitrate is effected by Asp. aureus and Asp. batatae, but not by Asp. Oryze A.<BR>(4) Neither nitrate nor nitrite is produced from the ammonium chloride by Asp. Oryzae A.<BR>(5) The nitrite is assimilated selectively by all three species in presence of the nitrate, which is attacked only after the complete consumption of the former.<BR>(6) The nitrite and the ammonium salt are consumed nearly at the same rate, although the former disappears somewhat earlier in the medium.<BR>(7) Small quantities of ammonia are always formed in old cultures.<BR>(8) Generally speaking, the nitrite may be regarded as an intermediate substance in the resorption of the nitrate by the moulds, especially by Asp. Oryzae A. The degree of the oxidation of the nitrite to the nitrate seems to vary in reference to the nature of the moulds.<BR>The authors wish to express their heartiest thanks to Professor Dr. T. Takahashi, for encouragement given them in the present work.

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