In vitro Evaluation of Small RNA Accumulation in Chrysanthemum Mediated by Chrysanthemum Stunt Viroid
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- TSURO Masato
- Faculty of Agriculture, Meijo University
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- SAKUMA Atsuko
- Faculty of Agriculture, Meijo University
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- MINO Masanobu
- Graduate School of Environmental Life Science, Kyoto Prefectural University
書誌事項
- タイトル別名
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- <i>In vitro</i> Evaluation of Small RNA Accumulation in Chrysanthemum Mediated by Chrysanthemum Stunt Viroid
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A comparative analysis of stunting was conducted at various temperatures to develop a simple and reliable method for evaluating the disease symptoms of the chrysanthemum stunt viroid (CSVd). CSVd-free plants had significantly greater plant height than CSVd-carrying plants at 22°C and 24°C. No significant differences in plant height were detected at high and low temperatures. These results suggest that the in vitro evaluation of plant growth at an appropriate temperature might provide a useful method for CSVd diagnosis. In addition, the intensity of the small RNA hybridization signal obtained by northern blot analysis using a full-length CSVd RNA as a probe indicated synchronous behavior that correlated with the degree of growth suppression. These results suggest that a small RNA from CSVd may lead directly to the suppression of plant growth, with a similar function to siRNAs in chrysanthemum.
収録刊行物
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- Environment Control in Biology
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Environment Control in Biology 51 (2), 95-98, 2013
日本生物環境工学会
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詳細情報 詳細情報について
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- CRID
- 1390001205259697024
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- NII論文ID
- 10031184275
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- NII書誌ID
- AA12029220
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- COI
- 1:CAS:528:DC%2BC3sXht1emtbrP
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- ISSN
- 18830986
- 1880554X
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- NDL書誌ID
- 024644443
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
- 使用不可