Effects of Medium Components and Shear Conditions on the Formation and Growth of Adventitious Bud Derived from Hairy Roots of <I>Atropa belladonna</I> L.
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- MERA Nobuaki
- Department of Biological Science and Technology, Tokai University
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- TAKAYAMA Shinsaku
- Department of Biological Science and Technology, Tokai University
Bibliographic Information
- Other Title
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- Effects of Medium Components and Shear Conditions on the Formation and Growth of Adventitious Bud Derived from Hairy Roots of Atropa belladonna L
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Abstract
The effects of culture conditions on the formation and growth of adventitious bud derived from hairy roots of Atropa belladonna L. were investigated. Various concentrations of kinetin (KN) alone were prepared in combination with Murashige and Skoog (MS) and Gamborg's B5 (B5) media. Although the number and weight of adventitious buds were increased in MS medium, no marked effects by KN were shown. In the effects of sucrose concentrations, the number and weight of adventitious buds were most increased at 1% sucrose in MS medium. In the effects of inorganic components in MS medium, the strengths of inorganic solutions were modified, but the results were almost the same as a whole. The strong effects of ammonium nitrogen and thiamine were assumed in comparison with B5 medium. For inhibition of clump formation by shear conditions, independent adventitious buds were observed from 100 rpm rotary speed. Under the highest speed at 140 rpm, whereas the weight of adventitious buds was decreased, we found that the number was most increased as a positive effect of shear stress. Furthermore, multiple shoots with roots were regenerated from minute adventitious buds, so the possibility of mass seedling production from hairy roots of A. belladonna was suggested.
Journal
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- Environment Control in Biology
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Environment Control in Biology 50 (4), 393-406, 2012
Japanese Society of Agricultural, Biological and Environmental Engineers and Scientists
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Details 詳細情報について
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- CRID
- 1390001205259941760
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- NII Article ID
- 10031136576
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- NII Book ID
- AA12029220
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- ISSN
- 18830986
- 1880554X
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- NDL BIB ID
- 024119184
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed