Use of stable isotope probing to identify bacterial species involved in CO₂ fixation in the water just below the oxycline of the meromictic Lake Suigetsu, Japan
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- Mori Yumi
- Department of Marine Bioscience, Fukui Prefectural University
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- Kataoka Takafumi
- Department of Marine Bioscience, Fukui Prefectural University
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- Kondo Ryuji
- Department of Marine Bioscience, Fukui Prefectural University
書誌事項
- タイトル別名
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- Use of stable isotope probing to identify bacterial species involved in CO<sub>2</sub> fixation in the water just below the oxycline of the meromictic Lake Suigetsu, Japan
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説明
In this study, stable isotope probing was used to identify a bacterial community involved with bicarbonate fixation in the water just below the oxycline of the meromictic Lake Suigetsu, Japan. Water samples were incubated with 13C-labeled bicarbonate under either light or dark conditions. The identity of active bicarbonate-fixing bacteria was revealed by 16S rRNA gene clone library analysis of 13C-labeled DNA fractions. Bacterial clones closely related to the green sulfur bacterial genus Chlorobium were detected under light conditions. Clone sequences phylogenetically affiliated with the genera Arcobacter and Desulfocapsa were also detected under light conditions. In the dark incubation, clones belonging to the class Epsilonproteobacteria were detected. Clone sequences belonging to a clade containing the genus Thiomicrospira were also retrieved from dark incubation samples. These results indicated that phylogenetically diverse bacteria might fix CO2 in the water below the oxycline of Lake Suigetsu.
収録刊行物
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- Plankton and Benthos Research
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Plankton and Benthos Research 10 (2), 111-120, 2015
日本プランクトン学会、日本ベントス学会
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詳細情報 詳細情報について
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- CRID
- 1390001205264540672
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- NII論文ID
- 130005076203
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- NII書誌ID
- AA12130745
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- ISSN
- 1882627X
- 18808247
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- NDL書誌ID
- 026500399
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- 本文言語コード
- en
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- 資料種別
- journal article
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- データソース種別
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- NDLサーチ
- Crossref
- CiNii Articles
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- OpenAIRE
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- 使用不可