Development of compound microsatellite markers in the toxic dinoflagellate Alexandrium catenella (Dinophyceae)

DOI Web Site 被引用文献1件 参考文献18件
  • NISHITANI GOH
    Harmful Algal Bloom Division, National Research Institute of Fisheries and Environment of Inland Sea
  • NAGAI SATOSHI
    Harmful Algal Bloom Division, National Research Institute of Fisheries and Environment of Inland Sea
  • MASSERET ESTELLE
    University Montpellier 2, Laboratoire Ecosystèmes Lagunaires (UMR-CNRS 5119, ECOLAG), cc93
  • LIAN CHUNLIAN
    Asian Natural Environmental Science Center, the University of Tokyo
  • YAMAGUCHI SANAE
    Harmful Algal Bloom Division, National Research Institute of Fisheries and Environment of Inland Sea
  • YASUDA NINA
    Graduate School of Information Science and Engineering, Tokyo Institute of Technology
  • ITAKURA SHIGERU
    Harmful Algal Bloom Division, National Research Institute of Fisheries and Environment of Inland Sea
  • GRZEBYK DANIEL
    University Montpellier 2, Laboratoire Ecosystèmes Lagunaires (UMR-CNRS 5119, ECOLAG), cc93
  • BERREBI PATRICK
    University Montpellier 2, Institut des Sciences de l'Evolution (UMR-CNRS 5554), cc065
  • SEKINO MASASHI
    Tohoku National Fisheries Research Institute

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  • Development of compound microsatellite markers in the toxic dinoflagellate <i>Alexandrium catenella</i> (Dinophyceae)

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In this study, we identified 9 polymorphic compound microsatellite markers in the toxic dinoflagellate Alexandrium catenella, which was isolated from Thau lagoon (France, Mediterranean Sea), using the compound microsatellite marker technique. These new microsatellites were characterized by screening DNA templates from 43 A. catenella clonal strains, which were collected from a seawater sample from Inokushi Bay (Oita Prefecture, Japan). These loci provide one class of highly variable genetic marker: the number of alleles ranged from 3 to 8, and the estimate of gene diversity varied between 0.285 and 0.762. We also analyzed the same 43 DNA samples using microsatellite markers previously identified for A. catenella, comparing the PCR amplification success, the number of alleles and gene diversity. These three parameters were not significantly different, showing that the compound microsatellite markers have the same potential to reveal A. catenella genetic structure. This simple and efficient method reduces the costs for developing SSR markers and for analyzing the genetic structure of populations, therefore, suggesting the effectiveness of applying this method to other species.

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