<i>In vivo</i> Imaging of Collagen Fiber Orientation with Rapid, Orthogonally Polarization-resolved SHG Microscopy

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  • TANAKA Yuji
    Graduate School of Engineering Science, Osaka University
  • HASE Eiji
    Graduate School of Advanced Technology and Science, University of Tokushima
  • FUKUSHIMA Shu-ichiro
    Graduate School of Engineering Science, Osaka University Graduate School of Medicine, Nara Medical University
  • ARAKI Tsutomu
    Graduate School of Engineering Science, Osaka University
  • YASUI Takeshi
    Graduate School of Engineering Science, Osaka University Institute of Technology and Science, University of Tokushima Graduate School of Medicine, Nara Medical University

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Other Title
  • 高速・直交偏光分解第2高調波発生光顕微鏡を用いたコラーゲン配向の<i>in vivo</i>イメージング
  • 高速・直交偏光分解第2高調波発生光顕微鏡を用いたコラーゲン配向のin vivoイメージング
  • コウソク ・ チョッコウ ヘンコウ ブンカイ ダイ2 コウチョウハ ハッセイ ヒカリ ケンビキョウ オ モチイタ コラーゲンハイコウ ノ in vivo イメージング

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Abstract

Polarization-resolved second-harmonic-generation (SHG) microscopy is a powerful tool to visualize distribution of collagen fiber orientation in tissue with little invasion. However, long image acquisition time, resulting from mechanical rotation of a half-wave plate, makes this microscopy easy to suffer from motion artifact of a sample and hence has limited its use to in vivo application. In this paper, we constructed rapid, polarization-resolved SHG microscopy by combination of an electro-optics-modulator-based polarization modulation with improved data acquisition method. The constructed SHG microscopy enables us to visualize orientation mapping of dermal collagen fiber in rat skin in vivo without influence of motion artifact. This microscope will open the door for in vivo measurement of collagen fiber orientation in tissue.

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