Efficient Formation of Embryonic Bodies by using TASCL with Enhanced Gas-liquid Permeability

DOI

Bibliographic Information

Other Title
  • 気液透過性を付与したTASCLによるhiPS胚様体の高効率生産

Abstract

This paper reports on mass production of embryonic bodies (EBs) of human iPS cells by using Tapered Stencil for Cluster Culture device combined with gas/liquid permeable membrane. The device has an array of tapered square microwells, 500μm on a side at the top. The top of the wall separating microwells forms knife-edge so that each cell falls into any one of the microwells. The surface is modified with hydrophilic polymer to prevent cell adhesion. Thus, the cells seeded on the device aggregate at the bottom sealed with porous membrane, and thus, the cells self-assemble into EBs in each microwell. Compared to the previous device with non-permeable bottom, viability of EB were improved by >200% in 6day culture.

Journal

Details 詳細情報について

  • CRID
    1390001205268204800
  • NII Article ID
    130005163942
  • DOI
    10.11239/jsmbe.53.s236_01
  • ISSN
    18814379
    1347443X
  • Text Lang
    ja
  • Data Source
    • JaLC
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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