SHG（第二高調波発生光）イメージの2次元自己相関解析に基づいたヒト真皮コラーゲン線維構造の定量化

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  • 小倉 有紀
    資生堂グローバルイノベーションセンター 徳島大学大学院先端技術科学教育部
  • 田中 佑治
    大阪大学大学院基礎工学研究科
  • 長谷 栄治
    徳島大学大学院先端技術科学教育部
  • 山下 豊信
    資生堂グローバルイノベーションセンター
  • 安井 武史
    大阪大学大学院基礎工学研究科 徳島大学大学院理工学研究部

書誌事項

タイトル別名
  • Quantitative Evaluation of Collagen Fiber Structure in Human Dermis Based on Two-Dimensional Auto-Correlation Analysis of SHG (Second Harmonic Generation) Image
  • SHG(ダイニ コウチョウハ ハッセイ ヒカリ)イメージ ノ 2ジゲン ジコ ソウカン カイセキ ニ モトズイタ ヒト シンピ コラーゲン センイ コウゾウ ノ テイリョウカ

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説明

<p>Collagen fibers in the dermis are closely related to skin aging because collagen is one of structural proteins and hence plays an important role to determine the morphology and mechanical properties of the skin. Although histological and/or biochemical studies have been used to reveal the age-related decrease and disorganization of dermal collagen fibers, such invasive methods cannot be used for simple assessment or serial monitoring of skin aging. Therefore, there is a considerable need for non-invasive assessment method of skin aging. Recently, second-harmonic-generation (SHG) microscopy has attracted attention for in situ visualization of human dermal collagen fibers. Due to its specific collagen selectivity without the need for staining, non-invasiveness, and high-resolution 3D imaging capability, collagen fiber structure can be visualized as high-contrast SHG image in situ. However, extraction of quantitative parameters reflecting the collagen fiber structure from SHG image presents difficulties. In this article, we applied two-dimensional auto-correlation analysis to SHG images of dermal collagen fibers in human cheek skin. The collagen fiber structure index obtained from the analysis decreased significantly with aging. Furthermore, we confirmed a moderate correlation between the collagen fiber structure index and skin elasticity measured by a Cutometer®.This correlation indicates that the dermal collagen fiber structure plays an important role to determine skin elasticity. In other words, the auto-correlation analysis of SHG image can be used for evaluation of skin elasticity from the viewpoint of dermal collagen. The proposed method will be a powerful tool in the fields of skin cosmetics and anti-aging dermatology.</p>

収録刊行物

  • 生体医工学

    生体医工学 55 (2), 97-102, 2017

    公益社団法人 日本生体医工学会

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