肝スフェロイド内部の微細構造を制御する技術の開発

DOI
  • 小島 伸彦
    横浜市立大学大学院　生命ナノシステム科学研究科

書誌事項

タイトル別名
  • Establishment of a method for engineering of microstructures in hepatic spheroids

この論文をさがす

説明

<p>The objective of this study was to establish a method enabling us to control microarchitectures of spheroids. We injected 1 μl of culture medium suspending Hep G2 cell and alginate hydrogel beads into a 3% methylcellulose medium. The beads were 20 μm in diameter. The injected cells and beads were rapidly aggregated in 10 min, and formed hybrid spheroids in 24 h. Microchannel structures were constructed by enzymatic digestion of the beads. In a different experiment, injection of Hep G2 cells and HUVECs resulted in forming microstructures by autonomous cellular rearrangement. Another type of micropattern was able to be obtained by injecting HUVECs and small-spheroids. Dissolved polymeric molecules like ECMs were also possible to be concentrated together with Hep G2 cells. The amount of polymeric molecules existing inside of the spheroids was tunable by changing its concentration. These data show that our method is useful to engineer microarchitectures of spheroids.</p>

収録刊行物

  • 生体医工学

    生体医工学 54Annual (28PM-Abstract), S348-S348, 2016

    公益社団法人 日本生体医工学会

詳細情報 詳細情報について

  • CRID
    1390001205269508224
  • NII論文ID
    130005285455
  • DOI
    10.11239/jsmbe.54annual.s348
  • ISSN
    18814379
    1347443X
  • 本文言語コード
    ja
  • データソース種別
    • JaLC
    • CiNii Articles
  • 抄録ライセンスフラグ
    使用不可

問題の指摘

ページトップへ