QUALITY OF MULTIPLE-TIME WASHED RED BLOOD CELLS

DOI Web Site 14 References
  • Kaneko Yuji
    Japanese Red Cross Blood Service Headquarters Central Blood Institute
  • Hirayama Junichi
    Japanese Red Cross Blood Service Headquarters Central Blood Institute
  • Onodera Hidekazu
    Japanese Red Cross Blood Service Headquarters Central Blood Institute
  • Koike Toshiyasu
    Japanese Red Cross Blood Service Headquarters Central Blood Institute
  • Iwama Akira
    Japanese Red Cross Blood Service Headquarters Central Blood Institute
  • Chatani Makoto
    Japanese Red Cross Kanto-Koshinetsu Block Blood Center
  • Shiba Masayuki
    Japanese Red Cross Blood Service Headquarters Central Blood Institute
  • Nagai Tadashi
    Japanese Red Cross Blood Service Headquarters Central Blood Institute Japanese Red Cross Kanto-Koshinetsu Block Blood Center
  • Satake Masahiro
    Japanese Red Cross Blood Service Headquarters Central Blood Institute
  • Tadokoro Kenji
    Japanese Red Cross Blood Service Headquarters

Bibliographic Information

Other Title
  • 複数回洗浄した洗浄赤血球製剤の品質

Search this article

Description

<p>Washed red blood cells (WRC) are effective for preventing nonhemolytic transfusion reactions. However, no report has evaluated the quality and residual plasma proteins in multiple-time washed RBC. We evaluated the quality of once, twice, or three-times washed RBC during the 72 hours after washing. Washed RBC were prepared by removing the supernatant of centrifuged RBC and adding saline. Twice or three-times washed RBC were prepared by repeatedly washing. The RBC recovery rate was more than 98% even for three-times washed RBC. Residual plasma protein, IgA, and haptoglobin (Hp) values were decreased by repeatedly washing RBC. pH was maintained at above 6.8 during the storage period. The MCV, 2.3-DPG and RBC osmotic fragility test did not significantly differ between the 3 groups. ATP was significantly decreased only in the three-times washed RBC group in the 72 hours after washing. Supernatant Hb values and hemolysis rates were higher in the multiple-times washed RBC groups than in the once-washed RBC group during the storage period, but the hemolysis rate did not increase above 0.3%. Although supernatant potassium values after washing increased in a time-dependent manner, there was no difference between the 3 groups. Residual plasma protein, IgA, and Hp levels were markedly decreased by multiple washings. The quality of RBC was well-maintained until the end of their shelf life after multiple washings.</p>

Journal

References(14)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top