APPROACH TO PERIPHERAL BLOOD STEM CELL COLLECTION BY MEDICAL TECHNOLOGISTS

  • Sasaki Jun
    Transfusion and Cell Therapy Center, Hakodate Municipal Hospital
  • Masauzi Nobuo
    Transfusion and Cell Therapy Center, Hakodate Municipal Hospital Department of Internal Medicine, Hakodate Municipal Hospital
  • Morita Akie
    Transfusion and Cell Therapy Center, Hakodate Municipal Hospital
  • Senoh Noriko
    Transfusion and Cell Therapy Center, Hakodate Municipal Hospital
  • Murata Noriaki
    Transfusion and Cell Therapy Center, Hakodate Municipal Hospital
  • Hasegawa Satoshi
    Cell and Biological Assay Center, Hakodate Municipal Hospital
  • Takahashi Kazuto
    Cell and Biological Assay Center, Hakodate Municipal Hospital
  • Mori Satoru
    Medical Engineer Room, Hakodate Municipal Hospital
  • Sakai Nobuhiro
    Medical Engineer Room, Hakodate Municipal Hospital
  • Itoh Shinichi
    Department of Internal Medicine, Hakodate Municipal Hospital
  • Ichiki Kazuhiko
    Department of Internal Medicine, Hakodate Municipal Hospital
  • Komatsu Yasuyuki
    Department of Internal Medicine, Hakodate Municipal Hospital
  • Tsutsumi Yutaka
    Department of Internal Medicine, Hakodate Municipal Hospital
  • Matsuno Kazuhiko
    The Department of Medical Laboratory Science, Graduate School of Health Science Hokkaido University

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Other Title
  • 当院臨床検査技師の末梢血幹細胞採取への取り組み

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Abstract

Medical engineers (MEs) in our hospital used to perform apheresis procedures for peripheral blood stem cells (PBSC), while medical technologists (MTs) counted CD34-positive cells (CD34+) and processed and froze them. However, MEs ceased to perform this task in January 2005, and apheresis procedures have been done by MTs since then. Collection on holidays and weekends became possible once the MTs had been trained, after the restructuring of operations.<br> Apheresis is done in a private room of the patient ward. The floor nurse performs the venopuncture and drip. Oxygen and suction are piped into the private room, and a monitor and emergency cart are also set up. There is a doctor on duty in the ward on holidays and weekends. The status and vital signs of the patient during the apheresis procedure are recorded on a special chart, which enhances the safety of the procedure.<br> From March 2002 to December 2003, 13 PBSC apheresis procedures were done on 6 patients by MEs. From January 2004 to October 2008, after the restructuring of operations, 41 PBSC apheresis procedures were done on 23 patients by MTs.<br> The number of apheresis procedures on holidays and weekends increased from 0% before to 19.5% of the total after the restructuring. The success rate, measured in terms of collecting more than the preservation target for CD34+ (2.0×106/kg), increased from 50% to 87%. There was no difference in the appearance of complications before and after restructuring.<br> MTs in our hospital have been performing PBSC apheresis procedures under various safety precautions and without incident since 2005. They have been praised for alleviating patient stress and anxiety during the apheresis procedure, and have made PBSC collection more efficient and economical.<br> These findings highlight the need for ongoing support for MTs with regard to all procedures associated with cell therapy.<br>

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