Cytokine Profile of Human Abdominal Aortic Aneurysm: Involvement of JAK/STAT Pathway
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- Ohno Tomokazu
- Division of Cardiovascular Surgery, Department of Surgery, Kurume University School of Medicine
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- Aoki Hiroki
- Cardiovascular Research Institute, Kurume University
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- Ohno Satoko
- Division of Cardiovascular Medicine, Department of Internal Medicine, Kurume University School of Medicine
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- Nishihara Michihide
- Division of Cardiovascular Medicine, Department of Internal Medicine, Kurume University School of Medicine
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- Furusho Aya
- Division of Cardiovascular Medicine, Department of Internal Medicine, Kurume University School of Medicine
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- Hiromatsu Shinichi
- Division of Cardiovascular Surgery, Department of Surgery, Kurume University School of Medicine
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- Akashi Hidetoshi
- Division of Cardiovascular Surgery, Department of Surgery, Kurume University School of Medicine
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- Fukumoto Yoshihiro
- Division of Cardiovascular Medicine, Department of Internal Medicine, Kurume University School of Medicine
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- Tanaka Hiroyuki
- Division of Cardiovascular Surgery, Department of Surgery, Kurume University School of Medicine
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説明
<p>Objective: Abdominal aortic aneurysm (AAA) is characterized by inflammation and destruction of normal tissue architecture. The present study aimed to evaluate the inflammatory signaling cascade by analyzing the cytokines of AAA tissue.</p><p>Materials and Methods: We analyzed the comprehensive cytokine secretion profiles of 52 cytokines from human AAA in four patients with AAA using fluorescent beads-based multiplex assay. Further, the effect of janus kinase (JAK) inhibition by pyridone 6 on cytokine profiles was also evaluated.</p><p>Results: Cytokine secretion profiles were found to be similar among the four patients. A high level of JAK/signal transducers and activator of transcription (STAT) pathway activity in AAA tissue in culture was maintained, which may be attributed to the secretion of endogenous JAK-activating cytokines. Inhibition of JAK by pyridone 6 resulted in the suppression of STAT3 phosphorylation and secretion of a subset of chemokines and JAK-activating cytokines. However, the inhibition of JAK had no effect on the secretion of matrix metalloproteinase (MMP)-2, MMP-9, or TGF-β family that is responsible for the metabolism of extracellular matrix.</p><p>Conclusion: The findings of the present study suggested that AAA tissue exhibits a stereotypical profile of cytokine secretion, where JAK/STAT pathway may play a role in regulating a subset of cytokines. Identification of such a cytokine profile may reveal potential diagnostic markers and therapeutic targets for AAA.</p>
収録刊行物
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- Annals of Vascular Diseases
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Annals of Vascular Diseases 11 (1), 84-90, 2018
Annals of Vascular Diseases 編集委員会