石油系炭化水素を対象とした発光バクテリアを用いた簡易土壌汚染評価手法の開発―抽出溶媒の選定および石油系炭化水素の毒性評価―

DOI Web Site 被引用文献2件 参考文献29件

書誌事項

タイトル別名
  • Development of Simplified Soil Pollution Evaluation Method using Luminous Bacteria for Petroleum Hydrocarbons-Selection of Extraction Solvent and Toxicity Evaluation of Petroleum Hydrocarbons-
  • セキユケイ タンカスイソ オ タイショウ ト シタ ハッコウ バクテリア オ モチイタ カンイ ドジョウ オセン ヒョウカ シュホウ ノ カイハツ チュウシュツ ヨウバイ ノ センテイ オヨビ セキユケイ タンカスイソ ノ ドクセイ ヒョウカ
  • Selection of Extraction Solvent and Toxicity Evaluation of Petroleum Hydrocarbons
  • 抽出溶媒の選定および石油系炭化水素の毒性評価

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抄録

It is demanded more convenient survey methods for soil pollution by oils and techniques for toxicity evaluation of petroleum hydrocarbons. The quantitative bioassay using luminous bacteria has not been established. The accumulation of data obtained under scientific condition setting is required.<BR>Firstly, methanol, ethanol and n-propanol which are both moderate hydrophilic and oleophilic, were examined whether they are adequate as the extraction solvent for soil polluted by oils. From the results in bioassay test using luminous bacteria, methanol was selected as the most suitable extraction solvent from them.<BR>Secondly, the acute toxicity evaluation and the detection limit of gasoline, heating oil, light oil, benzene, toluene, n-hexane and carbon disulfide were experimentally examined. The severity of toxicity on luminous bacteria by each 100 vol% of them (without solvent) except n-hexane was carbon disulfide > toluene > gasoline > benzene > methanol > heating oil > light oil. Although the toxicities of 100 vol% of heating oil, light oil and n-hexane were weaker than that of methanol, the use of methanol as the solvent improved the detection sensitivity for them and was possible to detect the toxicity of them by luminous bacteria. The appropriate reaction time for toxicity evaluation or screening may be 15 to 45 minutes. The severity of toxicity on luminous bacteria evaluated using methanol solvent was gasoline > n-hexane > heating oil > toluene > carbon disulfide > benzene > light oil. Also, this study showed potential for quantitatively evaluating in the ranges of 0.02 to 1 vol% of gasoline and heating oil, 0.1 to 5 vol% of light oil and carbon disulfide, 0.2 to 5 vol% of benzene, 0.1 to 1.0 of toluene and 0.05 to 0.5 vol% of n-hexane in methanol solvent (extractant).

収録刊行物

  • 資源と素材

    資源と素材 126 (12), 684-690, 2010

    一般社団法人 資源・素材学会

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