Chrysanthemum latifolium x C. grandiflorum cv. ‘Red Betty’ crossed to induce new cultivars: Hybrid genome characterization and species relationship analyzed by FISH and GISH

DOI 参考文献18件 オープンアクセス
  • Abd El-Twab Magdy Hussein
    Department of Botany and Microbiology, Faculty of Science, Minia University, El Minia City 61519, Egypt
  • Kondo Katsuhiko
    Laboratory of Plant Genetics and Breeding Science, Department of Agriculture, Faculty of Agriculture, Tokyo University of Agriculture, 1737 Funako, Atsugi City, Kanagawa Prefecture, 243-0034, Japan

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タイトル別名
  • <I>Chrysanthemum latifolium</I> x <I>C. grandiflorum</I> cv. ‘Red Betty’ crossed to induce new cultivars: Hybrid genome characterization and species relationship analyzed by FISH and GISH

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Hybridity, genome differentiation and relationship were investigated in an interspecific F1 hybrid (2n=45) artificially produced between Chrysanthemum latifolium (Shih) H. Ohashi et Yonek. (formerly: Ajania latifolia Shih) (2n=36) and C. grandiflorum Ramat cv. ‘Red Betty’ (2n=54) by FISH (fluorescent in situ hybridization) and GISH (genomic in situ hybridization) using 5S, 45S and total genomic DNA labeled probes. Seventy-three hybrid seedlings were successfully produced only in vitro. FISH on the chromosomes of the hybrid had 11 signals of 45S rDNA sites, while five and six signals of the 5S rDNA were detected. GISH distinguished approximately 27-yellow-colored chromosomes were hybridized with the probe of C. latifolium and 18 redcolored were not, which could be characteristics of C. grandiflorum cv. ‘Red Betty’. Nine out of the 27 chromosomes were common homologous chromosomes of the two genomes. The 18 red-colored chromosomes, which isolated by GISH without using blocking DNA, might come from a distantly related ancestor of C. latifolium.

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詳細情報 詳細情報について

  • CRID
    1390001205285078656
  • NII論文ID
    130004503230
  • DOI
    10.3199/iscb.9.7
  • ISSN
    18818285
    18815936
  • 本文言語コード
    en
  • データソース種別
    • JaLC
    • Crossref
    • CiNii Articles
    • OpenAIRE
  • 抄録ライセンスフラグ
    使用不可

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