Sol–gel preparation of apatite-coated silica macrospheres from water glass and their adsorption of bovine serum albumin and lysozyme

  • LI Jie
    The Graduate School of Natural Science and Technology, Okayama University
  • SHIROSAKI Yuki
    The Graduate School of Natural Science and Technology, Okayama University
  • HAYAKAWA Satoshi
    The Graduate School of Natural Science and Technology, Okayama University
  • STAMBOULIS Artemis
    School of Metallurgy and Materials, University of Birmingham
  • OSAKA Akiyoshi
    The Graduate School of Natural Science and Technology, Okayama University

Search this article

Abstract

Silica gel macrospheres were prepared from water glass as the silica source. The water glass solution was mixed with sodium alginate solution and added dropwisely to 1 M CaCl2 solution to form macrospheres with ∼4 and 2.6 mm in diameter. Those SiO2 macrospheres were soaked in 1:1 (volume) mixture of ethanol and 0.1 M Na2HPO4 to deposit hydroxyapatite layer (HAp-SiO2 macrospheres). Bovine serum albumin (BSA) and egg lysozyme (LYZ) were adsorbed those SiO2 and HAp-SiO2 macrospheres. The amount of proteins adsorbed was well correlated to the Langmuir-type adsorption equation. The electrostatic interaction was predominant between the macrosphere surface and the protein molecules. Protein molecular entrapment in the mesopores (5–25 nm) was also probable for the SiO2 macrospheres but not applicable to the HAp-SiO2 ones without the mesopores. From consideration that protein molecule has a few groups with negative or positive partial charges, a multi-layer adsorption model was proposed.

Journal

References(26)*help

See more

Details 詳細情報について

Report a problem

Back to top