Stimulation of glucoamylase gene expression with starch in Pholiota microspora
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- LI Yan
- The United Graduate School of Agricultural Sciences, Tottori University
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- KINJO Yukiko
- Faculty of Agriculture, Tottori University
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- WAN Jianing
- The United Graduate School of Agricultural Sciences, Tottori University
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- SHIMOMURA Norihiro
- Faculty of Agriculture, Tottori University
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- YAMAGUCHI Takeshi
- Faculty of Agriculture, Tottori University
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- AIMI Tadanori
- Faculty of Agriculture, Tottori University
Bibliographic Information
- Other Title
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- Pholiota microsporaにおけるグルコアミラーゼ遺伝子発現のデンプンによる促進
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Description
The glucoamylase gene (PnGlu1) from Pholiota microspora was amplified and characterized. The 1743-bp coding region of PnGlu1 encoded a polypeptide consisting of 581 amino acids with a signal peptide comprising 17 amino acids at the N-terminal. The deduced protein sequence has a two-domain structure consisting of an N-terminal domain with the glycoside hydrolase family 15 signatures and a C-terminal carbohydrate-binding module 20. Southern blot analysis revealed that only a single copy of PnGlu1 was present in the haploid genome of P. microspora. To investigate the capability of starch utilization in P. microspora, PnGlu1 expression level has been examined on minimal media containing different carbon sources such as glucose, soluble starch, corn starch, wheat starch, and potato starch. Quantitative reverse transcription-polymerase chain reaction showed that corn starch and soluble starch strongly stimulated the PnGlu1 gene expression of P. microspora.
Journal
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- Mushroom Science and Biotechnology
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Mushroom Science and Biotechnology 20 (2), 77-84, 2012
Japanese Society of Mushroom Science and Biotechnology
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Details 詳細情報について
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- CRID
- 1390001205288241536
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- NII Article ID
- 110009489219
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- NII Book ID
- AA12415767
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- ISSN
- 24327069
- 13487388
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- NDL BIB ID
- 023946715
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- CiNii Articles
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- Abstract License Flag
- Disallowed