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Pre-culture before Agrobacterium Infection to Leaf Segments and Meropenem Improves the Transformation Efficiency of Apple (Malus * domestica Borkh.)
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- Li Jijun
- The United Graduate School of Agricultural Science, Iwate University
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- Komori Sadao
- Faculty of Agriculture, Iwate University
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- Sasaki Ken
- Faculty of Agriculture, Iwate University
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- Mimida Naozumi
- Faculty of Agriculture, Iwate University
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- Matsumoto Shogo
- Graduate School of Bioagricultural Sciences, Nagoya University
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- Wada Masato
- Apple Research Station, National Institute of Fruit Tree Science
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- Soejima Junichi
- Apple Research Station, National Institute of Fruit Tree Science
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- Ito Yuji
- National Agricultural Research Center for Hokkaido Region
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- Masuda Tetsuo
- Apple Research Station, National Institute of Fruit Tree Science
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- Tanaka Norimitsu
- Faculty of Agriculture and Life Science, Hirosaki University
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- Shigeta Narumi
- Faculty of Agriculture, Iwate University
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- Watanabe Manabu
- The Field Science Center, Faculty of Agriculture, Iwate University
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- Suzuki Akira
- Faculty of Agriculture, Iwate University
Bibliographic Information
- Other Title
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- アグロバクテリウム感染に先立つ葉切片の前培養と Meropenem を用いたリンゴ（<i>Malus</i> × <i>domestica</i> Borkh.）の形質転換効率の向上
- Pre-culture before Agrobacterium infection to leaf segments and meropenem improves the transformation efficiency of apple (Malus×domestica Borkh.)
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Description
Transformation experiments in apple (Malus × domestica Borkh.) were conducted to improve the Agrobacterium-mediated transformation efficiency of many apple cultivars without using complicated procedures. A system of direct regeneration from leaf segments was used for these experiments. The effectiveness of meropenem hydrate (MEPM) as an antibiotic for Agrobacterium elimination was investigated for use of instead of cefotaxime sodium (CTX), which prevents shoot regeneration from apple leaf segments. Results show that 50 mg·L−1 of MEPM greatly enhanced the transformation efficiency. Experiments assessing the timing of kanamycin sulfate (KM) addition to the medium were also conducted to investigate the effective use of KM, the antibiotic used to select transformed cell. Addition of KM to the medium immediately after co-cultivation was completed is advantageous for the multiplication of transformed cells and for the induction of transformed shoots in spite of the low shoot regeneration rate. Based on knowledge of the timing of KM addition, the effects of pre-culture before Agrobacterium infection on the shoot regeneration medium were investigated. Pre-culture for 3–7 days improved the transformation efficiency. By applying both MEPM and the pre-culture, about 5% transformation efficiency in ‘Greensleeves’ was achieved on an apple cultivar without complicated procedures.<br>
Journal
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- Journal of the Japanese Society for Horticultural Science
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Journal of the Japanese Society for Horticultural Science 80 (3), 244-254, 2011
THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE
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Details 詳細情報について
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- CRID
- 1390001205289019904
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- NII Article ID
- 130004510717
- 40018904628
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- NII Book ID
- AA12177046
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- COI
- 1:CAS:528:DC%2BC3MXhtVGmt7jE
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- ISSN
- 1882336X
- 18823351
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- NDL BIB ID
- 11156008
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL Search
- Crossref
- CiNii Articles
- OpenAIRE
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- Abstract License Flag
- Disallowed