ナシにおける蛍光 <i>in situ</i> ハイブリダイゼーションによるリボゾーム RNA 遺伝子の検出
書誌事項
- タイトル別名
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- Detection of the Ribosomal RNA Gene in Pear (Pyrus spp.) using Fluorescence in situ Hybridization
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抄録
The locations of the 18S-5.8S-25S ribosomal RNA gene (rDNA) in the seedlings of Japanese pear [Pyrus pyrifolia (Burm. F.) Nakai] ‘Osa Gold’ and European pear (P. communis L.) ‘La France’ and the 18S ribosomal rDNA in ‘Osa Gold’ were determined using fluorescence in situ hybridization (FISH). 18S-5.8S-25S and 18S rDNA probes were labeled with biotin-16-dUTP. The probes were detected using a fluorescein isothiocyanate (FITC)-avidin conjugate with chromosomes counterstained with propidium iodide (PI). The 18S-5.8S-25S and 18S rDNA sites of ‘Osa Gold’ and the former of ‘La France’ were detected in telomeric positions of six chromosomes. The number and positions of rDNA sites were stable.<br>
収録刊行物
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- Journal of the Japanese Society for Horticultural Science
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Journal of the Japanese Society for Horticultural Science 79 (4), 335-339, 2010
一般社団法人 園芸学会
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詳細情報 詳細情報について
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- CRID
- 1390001205290238976
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- NII論文ID
- 40017325101
- 130004951614
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- NII書誌ID
- AA12177046
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- ISSN
- 1882336X
- 18823351
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- HANDLE
- 10232/18907
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- NDL書誌ID
- 10843140
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- IRDB
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可