Detection of the Ribosomal RNA Gene in Pear (Pyrus spp.) using Fluorescence in situ Hybridization
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- Yamamoto Masashi
- Faculty of Agriculture, Kagoshima University
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- Terakami Shingo
- National Institute of Fruit Tree Science
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- Yamamoto Toshiya
- National Institute of Fruit Tree Science
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- Takada Norio
- National Institute of Fruit Tree Science
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- Kubo Tatsuya
- Faculty of Agriculture, Kagoshima University
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- Tominaga Shigeto
- Faculty of Agriculture, Kagoshima University
Bibliographic Information
- Other Title
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- ナシにおける蛍光 <i>in situ</i> ハイブリダイゼーションによるリボゾーム RNA 遺伝子の検出
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Abstract
The locations of the 18S-5.8S-25S ribosomal RNA gene (rDNA) in the seedlings of Japanese pear [Pyrus pyrifolia (Burm. F.) Nakai] ‘Osa Gold’ and European pear (P. communis L.) ‘La France’ and the 18S ribosomal rDNA in ‘Osa Gold’ were determined using fluorescence in situ hybridization (FISH). 18S-5.8S-25S and 18S rDNA probes were labeled with biotin-16-dUTP. The probes were detected using a fluorescein isothiocyanate (FITC)-avidin conjugate with chromosomes counterstained with propidium iodide (PI). The 18S-5.8S-25S and 18S rDNA sites of ‘Osa Gold’ and the former of ‘La France’ were detected in telomeric positions of six chromosomes. The number and positions of rDNA sites were stable.<br>
Journal
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- Journal of the Japanese Society for Horticultural Science
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Journal of the Japanese Society for Horticultural Science 79 (4), 335-339, 2010
THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE
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Keywords
Details 詳細情報について
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- CRID
- 1390001205290238976
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- NII Article ID
- 40017325101
- 130004951614
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- NII Book ID
- AA12177046
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- ISSN
- 1882336X
- 18823351
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- HANDLE
- 10232/18907
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- NDL BIB ID
- 10843140
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- Text Lang
- en
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- Data Source
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- JaLC
- IRDB
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed