Analysis of Boron and B-pectin Complex Content in the Abscission Zone during Floral and Fruit Abscission in Tobacco (<i>Nicotiana tabacum</i>)
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- Tsukahara Keita
- Fuculty of Life and Environmental Sciences, University of Tsukuba
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- Iwai Hiroaki
- Fuculty of Life and Environmental Sciences, University of Tsukuba
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- Hamaoka Eri
- Fuculty of Life and Environmental Sciences, University of Tsukuba
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- Furukawa Jun
- Fuculty of Life and Environmental Sciences, University of Tsukuba
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- Matsunaga Toshiro
- NARO Agricultural Research Center
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- Satoh Shinobu
- Fuculty of Life and Environmental Sciences, University of Tsukuba
Bibliographic Information
- Other Title
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- タバコ落花および落果過程の離層におけるホウ素とホウ素–ペクチン複合体の存在量解析
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Abstract
After fruit development has been triggered by pollination, the abscission zone (AZ) in the pedicel strengthens its adhesion to retain the fruit. In tobacco, unpollinated flowers are shed from the AZ, while enlargement of the same tissue occurs in pollinated flowers. The abscission rate depends on effective pollination. In this study, we focused on pectin and boron (B) distribution and their functions in the AZ. B is an essential micronutrient that forms cross-links in pectin, which are important for organ differentiation and maturation. B content and the formation rate of the borate-ester cross-linked rhamnogalacturonan II dimer (dRG-II-B) were measured in tobacco. Expression of NtGUT1, which is important for the formation of dRG-II-B, and the B transporter NtNIP3;1 were analyzed in the peduncle and AZ. To determine B content and dRG-II-B formation rate, the following samples were collected: bud peduncle, bud AZ, fruit peduncle, fruit AZ, and flower AZs, which were treated to prevent pollination or were artificially pollinated. Samples treated in HNO3 were assayed for total B content with ICP-MS. Endo-polygalacturonase (EPG)-treated alcohol-insoluble residues were assayed for formation rates of dRG-II-B with HPLC. Although there was more total B in fruit AZs than in branches, no differences in total B, water-soluble B, and B relevant to cross-linking with RG-II were found between buds and fruits. The formation rates of dRG-II-B were also the same in peduncles and AZs in both buds and fruits. These have the same characteristics of variation with or without pollination. Expression of both NtGUT1 and NtNIP3;1 was highest in AZs of flowers before opening, however, expression of NtGUT1 in flower AZs without pollination was not significantly different from after artificial pollination. These results suggest that the presence of relatively large amounts of B and pectin is important in structural enhancement of AZs after anthesis, but is not related to abscission with or without pollination.<br>
Journal
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- Journal of the Japanese Society for Horticultural Science
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Journal of the Japanese Society for Horticultural Science 82 (1), 83-90, 2013
THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE
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Keywords
Details 詳細情報について
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- CRID
- 1390001205290796160
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- NII Article ID
- 130004510770
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- NII Book ID
- AA12177046
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- COI
- 1:CAS:528:DC%2BC3sXitl2iurw%3D
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- ISSN
- 1882336X
- 18823351
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- NDL BIB ID
- 024212725
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed