ATP を指標とした医療現場での洗浄評価判定法の検討

  • 土方 俊介
    スリーエム ヘルスケア株式会社 技術本部 感染管理製品技術部
  • 染谷 拓
    スリーエム ヘルスケア株式会社 医療用製品事業部 感染管理製品学術グループ
  • 木村 登
    スリーエム ヘルスケア株式会社 技術本部 感染管理製品技術部
  • 上林 裕始
    スリーエム ヘルスケア株式会社 技術本部 感染管理製品技術部

書誌事項

タイトル別名
  • Research on Practical Assessment of Medical Instrumentations Cleanliness in Healthcare Setting Using Adenosine Tri-phosphate as an Indicator
  • ATP オ シヒョウ ト シタ イリョウ ゲンバ デ ノ センジョウ ヒョウカ ハンテイホウ ノ ケントウ

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抄録

The Luciferin/Luciferase/Aadenosine Tri-Phosphate (ATP) bioluminescent method is commonly used for cleanliness verification of medical instruments. In Japanese Society of Medical Guideline for assessing the cleanliness of medical instrumentations in healthcare setting, the ATP measurement method is adopted as one of swabbing test methods. As the advantages of ATP method, high sensitivity, easy measurement and rapid readout capability are cited in this document. The guideline consecutively describes the ATP swabbing test method is not suitable to verify the cleanliness of narrow and lumen structure because it has limitation on access there. To solve this limitation of the ATP swab test method, we developed a test method of measuring residual soil from whole instrument surface using extract liquid. 3MTM Clean-TraceTM ATP Monitoring Systems of Water ATP liquid test (Product Code: H2O) and Luminometer (Product Code: NGi) were used. To verify the performance of the extract liquid, we investigated the influence of the extract liquid on the measurement result and the performance of the extract liquid. ATP stability of two types of commercially available preserved sheep blood (heparinized or Alsever solution added) were examined. A soil model device was prepared by applying mixture of sheep blood and 1.0% sulfate protamine to forceps box lock and drying. Using the selected extract liquid, the contaminant was extracted with ultrasonic cleaning process at 50 °C for 30 min and quantitatively thorough extraction was confirmed by protein measurement. ATP value of each diluents of sheep blood was measured, and the correlation between protein and ATP was evaluated.

収録刊行物

  • 医療機器学

    医療機器学 84 (3), 335-342, 2014

    一般社団法人日本医療機器学会

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