TOXICITY TESTING USING CONCHOSPORES AND THEIR GERMLINGS OF <i>PORPHYRA YEZOENSIS</i>

  • Takami Tohru
    社団法人 土木学会 宮崎大学大学院工学研究科システム工学専攻
  • Maruyama Toshiro
    社団法人 土木学会 宮崎大学 工学部土木環境工学科
  • Suzuki Yoshihiro
    社団法人 土木学会 宮崎大学 工学部土木環境工学科
  • Miura Akio
    青森大学 工学部生物工学科

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  • スサビノリの殻胞子と発芽体を用いた毒性試験法
  • スサビノリ ノ カクホウシ ト ハツガタイ オ モチイタ ドクセイ シケンホウ

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Abstract

The objective of this study is to establish the methodology of toxicity test of detrimental substances using conchospores and their germlings of Porphyra yezoensis. Release of conchospores from mature free-living conchocelis took place after 7 days when the culture transfered from 23°C to 15°C temperature conditions with 10hL:14hD in 1/20PES medium, and 80% of conchospores were released for first 3 hours when the light condition was changed from off to on in a day. As substrata to be adhered conchospores, the glass plate was the most adequate and concerning the method of adhering conchospores, natural sedimentation of conchospores onto the glass plate was the most adequate. Adhesion and survival ratios of conchospores were constant, even if the date and the number of collecting conchospores were different.

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