光応答性人工核酸を用いた遺伝子操作および遺伝子解析

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  • 藤本 健造
    北陸先端科学技術大学院大学マテリアルサイエンス研究科

書誌事項

タイトル別名
  • Photochemical DNA Manipulation and DNA Analysis by Photoresponsive Artificial DNA
  • ヒカリ オウトウセイ ジンコウ カクサン オ モチイタ イデンシ ソウサ オヨビ イデンシ カイセキ

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抄録

DNA engineering, the basis of modern protein engineering, has been developed since the 1960s with many discoveries, including those of various restriction enzymes and polymerases, and a number of Nobel laureates for their contributions to, for example, the PCR method or DNA sequencing method. The existing biotechnology is thus essential to basic researches in the present DNA analysis or biochemistry. While many methods for enzymatic and chemical ligation of DNA fragment via a native phosphodiester bond or non-native linkages have been demonstrated, there are only a few methods for photoinduced non-enzymatic chemical ligation. The merit of the photochemical ligation avoiding the need for additional reagent is obvious. Furthermore, their actions are controllable within space and time by the choice of proper irradiation methods. The photoligation methods can be used as a tool for DNA engineering and nanotechnology, and as photoregulated diagnostic and therapeutic agents. Thcrcforc, the targct in my research is the development about phototriggered DNA manipulation via intelligent nucleic acids toward for the next generation DNA manipulation.

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