Metabolic Studies of Amino Acids by Rat Liver Cells in Primary Culture

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  • KISHI Kyoichi
    Department of Nutrition School of Medicine, Tokushima University
  • FUJITA Yoshiaki
    Department of Nutrition School of Medicine, Tokushima University
  • TANAKA Keiji
    Institute for Enzyme Research, School of Medicine, Tokushima University
  • ICHIHARA Akira
    Institute for Enzyme Research, School of Medicine, Tokushima University

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Other Title
  • ラット肝初代培養細胞を用いた培地アミノ酸の代謝的研究
  • ラット カン ショダイ バイヨウ サイボウ オ モチイタ バイチ アミノサン

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Abstract

Isolated liver cells from adult rats were cultured for 24 hours in enriched medium (Williams medium E, WE) with 10% fetal bovine serum, and with or without various hormones and then the amino acid concentrations in the medium were measured. The concentrations of alanine, glycine, arginine and aspartate decreased very much, the decreases in alanine and glycine being markedly enhanced by the presence of glucagon. The concentrations of lysine, tyrosine, phenylalanine, threonine and methionine decreased moderately and dexamethasone stimulated that of tyrosine, particularly.<BR>On the other hand, the concentrations of isoleucine, leucine, proline, cystine, glutamate and histidine did not change significantly and those of serine, valine and ornithine increased.<BR>These results can mainly be explained by the metabolic activities of liver cells, particularly gluco-neogenesis for alanine and aspartate, ureogenesis for arginine and ornithine, and interconversion of serine and glycine and the inability of hepatocytes to metabolize branched chain amino acids. These explanations were supported more clearly by results using unsupplemented salt solution (Hanks' solution). In this medium the concentrations of amino acids released into the medium were very similar to those found in liver proteins, except for those of alanine, glutamate, aspartate, proline and arginine, which were much lower than in liver proteins.<BR>The present work also suggests that liver cells in primary culture retain various liver functions and thus are useful for studies on liver functions in vitro.

Journal

  • Eiyo To Shokuryo

    Eiyo To Shokuryo 31 (6), 571-577, 1978

    JAPAN SOCIETY OF NUTRITION AND FOOD SCIENCE

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