ガスクロマトグラフによる尿中サッカリンの微量分析法とヒトにおける排泄

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書誌事項

タイトル別名
  • Micro-determination of Saccharin in Urine by Gaschromatography and Its Excretion in Man
  • ガス クロマトグラフ ニヨル ニョウチュウ サッカリン ノ ビリョウ ブンセキ

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抄録

Saccharin has been used as a non-nutritious sweetener in the belief that it is not metabolized in the body and excreted in the urine in unchanged form.<BR>In this paper, a new gas chromatographic method for the micro-determination of saccharin in urine samples is described. The principle of this method is based on the conversion of saccharin to 1, 2-benzisothiazoline-1, 1-dioxide by hydrochloric acid and zinc at 90° and its gas-chromatographic determination (Flame Photometric Detector) after extraction with methylisobutyl ketone. By this method, saccharin in the normal urine samples collected from Kagawa prefecture in 1974, 1978, 1979 was shown to be in the range of 0-5, 963μg within 8hr. Saccharin in the urine samples from diabetic patients was shown to be in the range of 0-25, 600μg in 1980.<BR>When human subjects were received a single oral dose of 66mg saccharin sodium (50mg as saccharin), more than 90% of dose was recovered in the 96 hours-urine. More than 60% of saccharin was recovered in the first 6 hours.

収録刊行物

  • 栄養と食糧

    栄養と食糧 33 (6), 407-415, 1980

    公益社団法人 日本栄養・食糧学会

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