STUDIES ON FUCOSYLTRANSFERASES RELATED TO BIOSYNTHESIS OF HUMAN BLOOD GROUP SUBST ANCES

DOI Web Site Open Access
  • YAZAWA SHIN
    Department of Legal Medicine, School of Medicine, Gunma University

Bibliographic Information

Other Title
  • ヒトの血液型物質合成フコシルトランス フェラーゼに関する研究
  • ヒト ノ ケツエキガタ ブッシツ ゴウセイ フコシル トランスフェラーゼ ニカ
  • I. FUCOSYLTRANSFERASES IN HUMAN SALIVA
  • I. ヒト唾液のフコシルトランス フェラーゼについて

Search this article

Description

Focosyltransferases catalize the transfer of 14C-fucose from GDP-L-fucose-14C to oligosaccharides from human milk were demonstrated in human saliva.<BR>1) An α- (1→4) -fucosyltransferase that synthesizes lacto-N-fucopentaose II and lacto-N-difucohexaose I from lacto-N-tetraose and lacto-N-fucopentaose I, respectively, was detected in saliva samples of Le (a-b+) secretors and Le (a+b-) non-secretors in which Lea substance was secreted.<BR>This enzyme activity was not detectable in saliva samples of Le (a-b-) secretors and nonsecretors, in which Lea substance was absent.<BR>2) An α- (1→2) -fucosyltransferase that synthesizes lacto-N-fucopentaose I from lacto-N-tetraose, was detected in saliva samples from Le (a-b+) secretors, secreted H and Leb substances, and from Le (a-b-) secretors, secreted only H substance.<BR>3) An α- (1→3) -fucosyltransferase was detected in all saliva samples from donors of different ABO and Lewis blood groups irrespective of their ABH secretor status.<BR>4) The fucosyltransferases were activated by Mn++ or Mg++ ions, and was inhibited completely with ATP, GTP and EDTA. They had a broad pH optimum between 5.0 and 7.0.<BR>5) Lacto-N-difucohexaose I could be synthesized from lacto-N-fucopentaose I by theα- (1→4) -fucosyltransferase from a saliva of OLe (a-b+) sec.donor.The difucohexaose could not be synthesized from lacto-N-fucopentaose II by the α- (1→2) -fucosyltransferase. Concerning the substrate specificities of the enzymes, it was, therefore, confirmed that H antigens could be converted to Leb antigens.

Journal

Details 詳細情報について

Report a problem

Back to top