Effect of Lipid Addition on the Profile of Lipase Production by Mucor javanicus

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  • ムコール・ジャバニカスによるリパーゼの生産様相への脂質の添加効果
  • ムコール ジャバニカス ニ ヨル リパーゼ ノ セイサン ヨウソウ エ ノ シ

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Abstract

While increasing lipase production by Mucor javanicus IAM 6108, reduction in cultivation time and stabilization of lipase in the broth were investigated. The addition of 1 % soybean oil to the initial medium containing 3 % corn steep liquor, 1 % soluble starch, 0.2 % KH2PO4, 0.05 % MgSO4·7 H2O, 0.05 % KCl, and 1 % soybeanyuto reduced the cultivation time of lipase production from 50 h to 30h.<BR>Lipase activity in the broth decreased rapidly after reaching a maximum. To avoid this, the 1 % soybean oil was added to the broth at 25 h of cultivation before the maximum had been reached, As a result, maximum lipase activity was maintained for more than 10 h. This appeared due to the presence of fatty acids or triglycerides with double bonds on the broth.

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