The <I>erythro/threo</I> Separation of Non-trimethylsilylated Sphingolipid Bases by Normal Phase HPLC and GLC

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  • 順相HPLC及びGLCによるTMS化をしないスフィンゴシン塩基のエリトロ体とトレオ体との分離
  • ジュンソウ HPLC オヨビ GLC ニ ヨル TMSカ オ シナイ スフィン

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Abstract

The facile determination of erythro/threo ratios of sphingolipid bases without trimethylsilyl groups was made using synthetic Cn-dihydrosphingosines (1) (n=12, 14 and 16) as standard substances. The erythro/threo isomers of (1) could be separated on a normal phase HPLC column using N, O, O-triacetyl and N-acetyl derivatives of (1) [(2) and (3)], and on a GLC column (polyethylene glycol 20 M) using (2). The erythro content of (1) determined by the present methods showed good agreement, and with that by previously reported GLC methods using the trimethylsilyl derivatives of (1) and (3). Deuterium content of synthetic 2-d-Cn dihydrosphingosines can be determined using GC-MS based on the present GLC method.

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