高速液体クロマトグラフィーによるリン脂質組成の定量分析

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書誌事項

タイトル別名
  • Quantitative Analysis of Phospholipid Composition by High Performance Liquid Chromatography
  • コウソク エキタイ クロマトグラフィー ニ ヨル リン シシツ ソセイ ノ テ
  • Collaborative studies of the working group of JOCS
  • 日本油化学協会リン脂質専門委員会合同実験報告

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説明

A method for determining phospholipid composition was developed by ten laboratories working in collaboration. Commercially available soybean lecithin and egg yolk lecithin were used. Phospholipids were separated by high performance liquid chromatography using with a stainless steel column (φ 3 mm × 150250 mm) packed with silica gel (particle size 5 μ m) and UV detector (206 nm).<BR>Phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol and phosphatidic acid in soybean lecithin could be clearly separated with the mobile phase of hexane/isopropyl alcohol/0.2 M acetate buffer (pH 4.2) =8/8/1 (vol/vol).<BR>Sphingomyelin and lysophosphatidylcholine were also separated from the above four major phospholipids. The detector responce of these phospholipids for lipid phosphorus content showed good linearity.<BR>Repeatability and reproducibility shown by statistical analysis of the results indicated the present method to provide highly accurate results for major phospholipids of commercial lecithin.

収録刊行物

  • 油化学

    油化学 41 (12), 1171-1179, 1992

    公益社団法人 日本油化学会

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