High-Performance Liquid Chromatographic Analysis of Positional Isomers of Dipalmitoolein in Palm Oil as Halogen Adducts

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  • パーム油中のジパルミトオレインの位置異性体のハロゲン付加体としての高速液体クロマトグラフィー分析
  • パームユチュウ ノ ジパルミトオレイン ノ イチ イセイタイ ノ ハロゲン フ

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High performance liquid chromatographic separation of two positional triacylglycerol isomers of 1, 3-dipalmitoy1-2-oleoylglycerol (POP) and 1, 2-dipalmitoyl-3-oleoyl-rac-glycerol (PPO) as bromine or ICl adducts was carried out on a reversed stationary phase column packed with 3 μm particles of LiChrospher 100CH-18. Good separation of the triacylglycerol positional isomers required about 130 min as the bromine adduct using acetone/acetonitrile (8 : 2 vol/vol) as the mobile phase. Palm oil was analyzed by this method under similar conditions and the proportion of PPO to POP in palm oil was determined.

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