Nucleic Acid Reaction Chemistry to Clarify the Fluctuating Quality and Quantity of Nucleic Acids

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  • Okamoto Akimitsu
    Research Center for Advanced Science and Technology, The University of Tokyo

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  • 変動する核酸の質と量を明らかにする核酸反応化学
  • ヘンドウ スル カクサン ノ シツ ト リョウ オ アキラカ ニ スル カクサン ハンノウ カガク

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Abstract

The development of a chemical reaction for the detection of one epigenetic modification in a long nucleic acid is a chemically and biologically challenging research subject. Here we review three topics on chemical detection of methylation/demethylation process in nucleic acids; (1) sequence-specific osmium oxidation of DNA 5-methylcytosine; (2) DNA 5-hydroxymethylcytosine-specific oxidation with peroxotungstate; (3) capturing activated RNA demethylase complexes with specific RNA m6A recognition. These novel concepts of methylation/demethylation-specific reactions, based on straightforward chemical approaches, expand the range of methods available for the analysis of epigenetic modifications in nucleic acids.

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