STUDIES ON THE PATHOGENIC ORGANISM OF THE HYUGANETSU DISEASE

DOI
  • NAKAGAWA YOH
    Department of Microbiology, Kurume University School of Medicine
  • KANDA YUKISHIGE
    Department of Microbiology, Kurume University School of Medicine
  • MARUOKA MASAO
    Department of Microbiology, Kurume University School of Medicine

Bibliographic Information

Other Title
  • 日向熱病原体に関する研究
  • (I) CULTIVATION OF THE NAKAZAKI STRAIN IN YOLK SACS AND CULTIVATION IN TISSUES OF WHOLE EMBRYONIC MICE
  • 第1報 中崎株の孵化鷄卵々黄嚢内培養並にマウス全胎児組織えの培養

Description

The pathogenic organism of the Hyuganetsu disease, regarded as infectious mononucleosis, was isolated in Japan. However, many doubts remain in that the pathogenic organism is the causative agent of real infectious mononucleosis. On the other hand, the Tsutsugamushi disease seems to be included in the Hyuganetsu disease. The present authors have made investigations concerning the cultivation of the Nakazaki strain, which was isolated as the causative agent of the Hyuganetsu disease, in yolk sacs and the cultivation in tissues of whole embryonic mice. These results are obtained as follows:<br>1) The Nakazaki strain was successfully cultivated in yolk sacs only up to the 4th passage. 2) Cultivation in tissues of whole embryonic mice was successfully conducted. The infectivity of tissues increased to approximately 100-1000 times after 15 days of cultivation and the cultivation in vitro was successfully continued during at least 30 days by two passages.<br>3) At this time, the addition of hyaluronidase in the materials for inoculation promoted the establishment of infection and the cultivation was accelerated by this addition. 4) The pathogenic organisms propagated in the cytoplasma of cultivated tissues were either coccoid or oval in shape, approximately 0.3-0.4μ in diameter, under the photomicroscope. There was no remarkable variation compared with that of prepared from the living body.

Journal

  • VIRUS

    VIRUS 6 (6), 471-477, 1956

    The Japanese Society for Virology

Details 詳細情報について

  • CRID
    1390001205343614976
  • NII Article ID
    130003854318
  • DOI
    10.2222/jsv1951.6.471
  • ISSN
    18843425
  • Text Lang
    ja
  • Data Source
    • JaLC
    • Crossref
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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