Eukaryotic communities in wastewater treatment processes as revealed by 18S rRNA gene clone library
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- MATSUNAGA Kengo
- Dept. of Civil and Environmental Engineering, Tohoku University
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- KUBOTA Kengo
- Dept. of Civil and Environmental Engineering, Tohoku University
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- VONASEK Erica
- Dept. of Civil and Environmental Engineering, Tohoku University
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- TAKEMURA Yasuyuki
- Dept. of Civil and Environmental Engineering, Tohoku University
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- HARADA Hideki
- Dept. of Civil and Environmental Engineering, Tohoku University
Bibliographic Information
- Other Title
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- 18S rRNA遺伝子情報を用いた排水処理汚泥の真核生物群集構造解析
- 18S rRNA イデンシ ジョウホウ オ モチイタ ハイスイ ショリ オデイ ノ シンカク セイブツ グンシュウ コウゾウ カイセキ
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Abstract
Eukaryotic communities in two different wastewater processes, a full-scale anaerobic/aerobic activated sludge process and a pilot-scale Down-flow hanging sponge (DHS) process, was evaluated by constrcting 18S rRNA gene clone library. The clones close to the member of Opercularia was found to be a dominant eukaryote in anaerobic and aerobic activated sludges, whereas the clones affiliated to phylum Arthropoda were the most predominant in the DHS sludges. Eukaryotic communities of the activated sludges as revealed by 18S rRNA gene clone library were in agreement with published data determined by microscopic observation. On the other hand, eukaryotic communities as revealed by 18S rRNA gene clone library and microscopic observation were significantly different in the case of the DHS sluges; therefore, it was suggested that both molecular and microscopic approaches need to be conducted. The molecular approach can also reveal unidentified eukaryotic gene sequences, which might be important for understanding wastewater treatment processes.
Journal
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- Journal of Japan Society of Civil Engineers, Ser. G (Environmental Research)
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Journal of Japan Society of Civil Engineers, Ser. G (Environmental Research) 68 (7), III_13-III_19, 2012
Japan Society of Civil Engineers