書誌事項
- タイトル別名
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- Quantification of enteric viruses and microbial indicators in Ishinomaki coastal area
- シンサイ ゴ ノ イシノマキ エンガンイキ ニ オケル チョウカンケイ ウイルス オヨビ シヒョウ ビセイブツ ノ ケンシュツ ト ケンシュツ ソガイ ヒョウカ
この論文をさがす
説明
Waterborne and oyster-borne diseases are great concerns in Ishinomaki coastal area because a wastewater treatment plant was unable to utilize activated sludge process after the tsunami. In this study, we detected several kinds of enteric viruses, which are one of the most probable causes of the infection, and microbial indicators in quantitative manner. Two virus concentration methods, i.e., a novel large volume method and a conventional small volume method, were performed for a comparison purpose. Efficiencies in each of nucleic acid extraction and RT-qPCR were evaluated by internal controls. Aichi viruses and GII- and GIII-F-specific RNA phages were dominantly detected. GI- and GII-noroviruses and sapoviruses, causative agents of viral gastroenteritis, were also found. Viruses and indicators seemed to diffuse from wastewater treatment plant in both horizontal and vertical directions. The large volume virus concentration method gave higher positive ratio than the small volume method. However, the virus detection was more likely to be affected by RT-PCR inhibition. High molecular weight (>10 kDa) organic compounds which absorb 254 nm ultraviolet were assumed to be causes of the inhibition. We demonstrated distributions of pathogenic viruses in Ishinomaki coastal area as well as need for methods to relieve RT-PCR inhibition efficiently.
収録刊行物
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- 土木学会論文集Ｇ（環境）
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土木学会論文集Ｇ（環境） 68 (7), III_285-III_296, 2012
公益社団法人 土木学会
