Simple Modified Method for Fungal Slide Preparation

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  • ポリプロピレン製小物収納用ケースを用いた簡便なスライド培養法
  • ポリプロピレンセイ コモノ シュウノウヨウ ケース オ モチイタ カンベン ナ スライド バイヨウホウ

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Abstract

  Slide culture preparations are necessary for accurately identifying dermatophytes. Because the standard slide culture technique is difficult for dermatologists to carry out, there is a need for an economical and easy-to-prepare system without special equipment. We placed a block of agar medium on a square cover glass (24 × 24mm) in the bottom of a case made of polypropylene (Free case, PK-201, HINODEWASHI CO., LTD. Japan). The agar block inoculated with fungi was overlaid with another cover glass on top of it. A plastic petri dish containing 4 cases as described above was sealed with adhesive plastic tape and incubated at 27°C.

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