Carbon and Nitrogen stable isotope analysis by EA/IRMS

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  • 元素分析/同位体比質量分析計(EA/IRMS)を用いた炭素・窒素安定同位体比の測定方法とその応用
  • ゲンソ ブンセキ ドウイタイヒ シツリョウ ブンセキケイ EA IRMS オ モチイタ タンソ チッソ アンテイ ドウイタイヒ ノ ソクテイ ホウホウ ト ソノ オウヨウ

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Abstract

Continuous-flow (on-line) system has revolutionized the study of natural stable isotope variations in organic matter and organic compounds. In particular, carbon and nitrogen isotope analysis of bulk samples using elemental analyzer/isotope ratio mass spectrometer (EA/IRMS) has been widely used in various field of studies, which allows a simple and rapid analysis of the isotope ratios for all types of organic and a wide range of inorganic samples, with a small amount of sample materials compared to the traditional off-line Dual-Inlet method. However, stability, accuracy, and precision on the observed isotope ratios strongly reflect various factors associated with continuous-flow system, such as peak intensity (i.e., sample weight), memory effect, and He dilution ratio. Unfortunately, the complete removal of such uncertainties should not be realistic, particularly in routine isotope measurements of a great number of samples. Here, we review the principles of stable isotope analysis on the traditional off-line Dual-Inlet and continuous-flow EA/IRMS methods, and also show an applicable calibration and standardization sequence. We hope that this paper is useful for routine application of EA/IRMS technique to various studies in geochemistry, ecology, biology, food science, and other area of sciences.

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