微量湿式分析による分子レベル同位体比の品質管理と確度向上 : 特に天然存在比の正確な評価とStable Isotope Probing(SIP)法の応用に向けて
書誌事項
- タイトル別名
-
- Quality control by wet chemical pre-treatment for precise compound-specific isotope analysis
- ビリョウ シッシキ ブンセキ ニ ヨル ブンシ レベル ドウイタイヒ ノ ヒンシツ カンリ ト カクド コウジョウ トクニ テンネン ソンザイヒ ノ セイカク ナ ヒョウカ ト Stable Isotope Probing SIP ホウ ノ オウヨウ ニ ムケテ
この論文をさがす
抄録
Recent advances in the application of molecular approaches have emphasized our potentially underestimate of microbial diversity in natural environments and have revealed comprehensive biogeochemical processes. One of the biggest challenges for organic geochemists and/or microbial ecologists is to identify which organisms are carrying out a specific set of metabolic processes. To answer this fundamental question, stable isotope probing (SIP) together with compound-specific isotope analysis (CSIA) is a useful technique to understand the microbial ecology and its biogeochemical cycles. The method relies on the incorporation of a substrate that is highly enriched in a stable isotope such as 13C and15N, and the identification of specific molecular targets through active microorganisms in any environment. However, we have to pay attention about highly SI-enriched contamination problems and co-elution problems during pretreatment of chemical analysis and also in chromatographic procedures, which potentially prevent us from performing precise CSIA. Here, we point out quality control by wet chemical pre-treatment for precise compound-specific isotope analysis by isotope-ratio mass spectrometry coupled to gas chromatography and liquid chromatography. Not only in biogeochemical background but also from a scope of common welfare, CSIA technique is also widely useful tools for validation approaches in food material, anti-doping test, and environmental assessment. For further precise CSIA, we also discuss representative optimized analytical conditions of some model compounds including intact polar lipids (IPLs) with its derivatives, n-alkane, and amino acids.
収録刊行物
-
- Researches in Organic Geochemistry
-
Researches in Organic Geochemistry 26 (0), 81-93, 2010
日本有機地球化学会
- Tweet
詳細情報 詳細情報について
-
- CRID
- 1390001205414185600
-
- NII論文ID
- 110008006525
- 20000583343
-
- NII書誌ID
- AA11889471
-
- ISSN
- 21897891
- 13449915
-
- NDL書誌ID
- 10958333
-
- 本文言語コード
- ja
-
- データソース種別
-
- JaLC
- NDL
- CiNii Articles
- KAKEN
-
- 抄録ライセンスフラグ
- 使用不可